Many clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-
associated protein 9 (Cas9)-based genome editing technologies take advantage of Cas …

In most crop breeding programs, the rate of yield increment is insufficient to cope with the
increased food demand caused by a rapidly expanding global population. In plant breeding …

Induced pluripotent stem cell (iPSC)-derived organoids provide models to study human
organ development. Single-cell transcriptomics enable highly resolved descriptions of cell …

Self-organizing neural organoids grown from pluripotent stem cells,–combined with single-
cell genomic technologies provide opportunities to examine gene regulatory networks …

Abstract Homology-directed repair (HDR), a method for repair of DNA double-stranded
breaks can be leveraged for the precise introduction of mutations supplied by synthetic DNA …

Genome editing technologies operate by inducing site-specific DNA perturbations that are
resolved by cellular DNA repair pathways. Products of genome editors include DNA breaks …

Eukaryotic cells deploy overlap** repair pathways to resolve DNA damage.
Advancements in genome editing take advantage of these pathways to produce permanent …

Precise gene editing is—or will soon be—in clinical use for several diseases, and more
applications are under development. The programmable nuclease Cas9, directed by a …

Investigations of CRISPR gene knockout editing profiles have contributed to enhanced
precision of editing outcomes. However, for homology-directed repair (HDR) in particular …

The first step in CRISPR-Cas9-mediated genome editing is the cleavage of target DNA
sequences that are complementary to so-called spacer sequences in CRISPR guide RNAs …