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Deep tissue two-photon microscopy
With few exceptions biological tissues strongly scatter light, making high-resolution deep
imaging impossible for traditional—including confocal—fluorescence microscopy. Nonlinear …
imaging impossible for traditional—including confocal—fluorescence microscopy. Nonlinear …
Nonlinear magic: multiphoton microscopy in the biosciences
Multiphoton microscopy (MPM) has found a niche in the world of biological imaging as the
best noninvasive means of fluorescence microscopy in tissue explants and living animals …
best noninvasive means of fluorescence microscopy in tissue explants and living animals …
Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms
Although the nonlinear optical effect known as second-harmonic generation (SHG) has
been recognized since the earliest days of laser physics and was demonstrated through a …
been recognized since the earliest days of laser physics and was demonstrated through a …
Principles of two-photon excitation microscopy and its applications to neuroscience
The brain is complex and dynamic. The spatial scales of interest to the neurobiologist range
from individual synapses (∼ 1 μm) to neural circuits (centimeters); the timescales range from …
from individual synapses (∼ 1 μm) to neural circuits (centimeters); the timescales range from …
Three-dimensional high-resolution second-harmonic generation imaging of endogenous structural proteins in biological tissues
We find that several key endogenous protein structures give rise to intense second-
harmonic generation (SHG)—nonabsorptive frequency doubling of an excitation laser line …
harmonic generation (SHG)—nonabsorptive frequency doubling of an excitation laser line …
Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence
Multiphoton microscopy relies on nonlinear light–matter interactions to provide contrast and
optical sectioning capability for high-resolution imaging. Most multiphoton microscopy …
optical sectioning capability for high-resolution imaging. Most multiphoton microscopy …
Membrane imaging by second-harmonic generation microscopy
We present a detailed analysis of the generation of second-harmonic radiation from
biological membranes labeled with a styryl dye. In particular, we consider the high …
biological membranes labeled with a styryl dye. In particular, we consider the high …
[HTML][HTML] Imaging coronary artery microstructure using second-harmonic and two-photon fluorescence microscopy
The microstructural basis for the mechanical properties of blood vessels has not been
directly determined because of the lack of a nondestructive method that yields a three …
directly determined because of the lack of a nondestructive method that yields a three …
[ספר][B] Second-order nonlinear optical characterization techniques: an introduction
T Verbiest, K Clays, V Rodriguez - 2009 - taylorfrancis.com
Although chemists, biochemists, biologists, and material scientists are often interested in
using nonlinear optical techniques for characterizing their samples, they seldom have the …
using nonlinear optical techniques for characterizing their samples, they seldom have the …
Second harmonic generation imaging of endogenous structural proteins
We show that structural protein arrays consisting largely of collagen, myosin, and tubulin,
and their associated proteins can be imaged in three dimensions with high contrast and …
and their associated proteins can be imaged in three dimensions with high contrast and …