Fluorescent proteins and their applications in imaging living cells and tissues

DM Chudakov, MV Matz, S Lukyanov… - Physiological …, 2010 - journals.physiology.org
Green fluorescent protein (GFP) from the jellyfish Aequorea victoria and its homologs from
diverse marine animals are widely used as universal genetically encoded fluorescent labels …

Photoinduced chemistry in fluorescent proteins: curse or blessing?

A Acharya, AM Bogdanov, BL Grigorenko… - Chemical …, 2017 - ACS Publications
Photoinduced reactions play an important role in the photocycle of fluorescent proteins from
the green fluorescent protein (GFP) family. Among such processes are photoisomerization …

Photoswitchable fluorescent proteins enable monochromatic multilabel imaging and dual color fluorescence nanoscopy

M Andresen, AC Stiel, J Fölling, D Wenzel… - Nature …, 2008 - nature.com
Fluorescent proteins that can be reversibly photoswitched between a fluorescent and a
nonfluorescent state are important for innovative microscopy schemes, such as protein …

Structural basis for reversible photoswitching in Dronpa

M Andresen, AC Stiel, S Trowitzsch, G Weber… - Proceedings of the …, 2007 - pnas.org
Dronpa is a novel GFP-like fluorescent protein with exceptional light-controlled switching
properties. It may be reversibly switched between a fluorescent on-state and a …

Lens-based fluorescence nanoscopy

C Eggeling, KI Willig, SJ Sahl, SW Hell - Quarterly reviews of …, 2015 - cambridge.org
The majority of studies of the living cell rely on capturing images using fluorescence
microscopy. Unfortunately, for centuries, diffraction of light was limiting the spatial resolution …

Fluorescent proteins: shine on, you crazy diamond

P Dedecker, FC De Schryver… - Journal of the American …, 2013 - ACS Publications
In this Perspective we discuss recent trends in the development and applications of
fluorescent proteins. We start by providing a historical and structural perspective of their …

1.8 Å bright-state structure of the reversibly switchable fluorescent protein Dronpa guides the generation of fast switching variants

AC Stiel, S Trowitzsch, G Weber, M Andresen… - Biochemical …, 2007 - portlandpress.com
RSFPs (reversibly switchable fluorescent proteins) may be repeatedly converted between a
fluorescent and a non-fluorescent state by irradiation and have attracted widespread interest …

[HTML][HTML] Single-molecule fluorescence photoswitching: Design and synthesis of photoswitchable fluorescent molecules

T Fukaminato - Journal of Photochemistry and Photobiology C …, 2011 - Elsevier
Single-molecule fluorescence photoswitching: Design and synthesis of photoswitchable
fluorescent molecules - ScienceDirect Skip to main contentSkip to article Elsevier logo …

Photoswitches: Key molecules for subdiffraction‐resolution fluorescence imaging and molecular quantification

M Heilemann, P Dedecker, J Hofkens… - Laser & Photonics …, 2009 - Wiley Online Library
Optical microscopes, often referred to as 'light microscopes', use visible light and a system of
lenses to provide us with magnified images of small samples. Combined with highly …

GFP family: structural insights into spectral tuning

AA Pakhomov, VI Martynov - Chemistry & biology, 2008 - cell.com
Proteins homologous to green fluorescent protein (GFP) span most of the visible spectrum,
offering indispensable tools for live cell imaging. Structural transformations, such as …