Understanding how chromatin is folded in the nucleus is fundamental to understanding its
function. Although 3D genome organization has been historically difficult to study owing to a …

Rapidly develo** technologies have recently fueled an exciting era of discovery in the
field of chromosome structure and nuclear organization. In addition to chromosome …

Acquisition of cell fate is thought to rely on the specific interaction of remote cis-regulatory
modules (CRMs), for example, enhancers and target promoters. However, the precise …

As in eukaryotes, bacterial genomes are not randomly folded. Bacterial genetic information
is generally carried on a circular chromosome with a single origin of replication from which …

The sequence of a genome is insufficient to understand all genomic processes carried out in
the cell nucleus. To achieve this, the knowledge of its three-dimensional architecture is …

The Sc2. 0 project is building a eukaryotic synthetic genome from scratch. A major milestone
has been achieved with all individual Sc2. 0 chromosomes assembled. Here, we describe …

Here, we report the design, construction, and characterization of a tRNA neochromosome, a
designer chromosome that functions as an additional, de novo counterpart to the native …

Structural variants (SVs) can result in changes in gene expression due to abnormal
chromatin folding and cause disease. However, the prediction of such effects remains a …

Chromosomes of a broad range of species, from bacteria to mammals, are structured by
large topological domains whose precise functional roles and regulatory mechanisms …

Transcription generates local topological and mechanical constraints on the DNA fiber,
leading to the generation of supercoiled chromosome domains in bacteria. However, the …