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Fusion tags for protein solubility, purification and immunogenicity in Escherichia coli: the novel Fh8 system
Proteins are now widely produced in diverse microbial cell factories. The Escherichia coli is
still the dominant host for recombinant protein production but, as a bacterial cell, it also has …
still the dominant host for recombinant protein production but, as a bacterial cell, it also has …
Effective production of human growth factors in Escherichia coli by fusing with small protein 6HFh8
YS Kim, HJ Lee, M Han, N Yoon, Y Kim, J Ahn - Microbial Cell Factories, 2021 - Springer
Abstract Background Growth factors (GFs) are signaling proteins that affect cellular
processes such as growth, proliferation, and differentiation. GFs are used as …
processes such as growth, proliferation, and differentiation. GFs are used as …
Efficient sortase-mediated ligation using a common C-terminal fusion tag
SA Reed, DA Brzovic, SS Takasaki… - Bioconjugate …, 2020 - ACS Publications
Sortase-mediated ligation is a powerful method for generating site-specifically modified
proteins. However, this process is limited by the inherent reversibility of the ligation reaction …
proteins. However, this process is limited by the inherent reversibility of the ligation reaction …
Double‐stranded RNA targeting calmodulin reveals a potential target for pest management of Nilaparvata lugens
W Wang, P Wan, F Lai, T Zhu… - Pest Management …, 2018 - Wiley Online Library
BACKGROUND Calmodulin (CaM) is an essential protein in cellular activity and plays
important roles in many processes in insect development. RNA interference (RNAi) has …
important roles in many processes in insect development. RNA interference (RNAi) has …
The Fh8 tag: a fusion partner for simple and cost-effective protein purification in Escherichia coli
Downstream processing is still a major bottleneck in recombinant protein production
representing most of its costs. Hence, there is a continuing demand of novel and cost …
representing most of its costs. Hence, there is a continuing demand of novel and cost …
High-level production of N-terminal pro-brain natriuretic peptide, as a calibrant of heart failure diagnosis, in Escherichia coli
YS Kim, N Karisa, WY Jeon, H Lee, Y Kim… - Applied Microbiology and …, 2019 - Springer
Heart failure (HF) is a coronary disease that affects people worldwide and has a high
mortality rate. N-terminal pro-brain natriuretic peptide (NT-proBNP) has been proven to be a …
mortality rate. N-terminal pro-brain natriuretic peptide (NT-proBNP) has been proven to be a …
Molecular and biochemical characterization of calmodulin from Echinococcus granulosus
N Wang, X Zhong, X Song, X Gu, W Lai, Y **e… - Parasites & vectors, 2017 - Springer
Background Echinococcus granulosus is a harmful cestode parasite that causes cystic
echinococcosis in humans as well as various livestock species and wild animals …
echinococcosis in humans as well as various livestock species and wild animals …
Cloning, expression, and characterization of a novel Opisthorchis viverrini calcium-binding EF-hand protein
A novel 22.8 kDa of Opisthorchis viverrini (Ov) calcium-binding EF-hand protein (Ov CaBP)
was identified and isolated from an immunoscreening of the adult stage Ov cDNA library by …
was identified and isolated from an immunoscreening of the adult stage Ov cDNA library by …
FhCaBP3: a Fasciola hepatica calcium binding protein with EF-hand and dynein light chain domains
S Banford, O Drysdale, EM Hoey, A Trudgett… - Biochimie, 2013 - Elsevier
A DNA sequence encoding a protein with predicted EF-hand and dynein light chain binding
domains was identified in a Fasciola hepatica EST library. Sequence analysis of the …
domains was identified in a Fasciola hepatica EST library. Sequence analysis of the …
[HTML][HTML] High yield production of human invariant chain CD74 constructs fused to solubility-enhancing peptides and characterization of their MIF-binding capacities
The HLA class II histocompatibility antigen gamma chain, also known as HLA-DR antigen-
associated invariant chain or CD74, has been shown to be involved in many biological …
associated invariant chain or CD74, has been shown to be involved in many biological …