A guide to sample delivery systems for serial crystallography

FZ Zhao, B Zhang, EK Yan, B Sun, ZJ Wang… - The FEBS …, 2019 - Wiley Online Library
Crystallography has made a notable contribution to our knowledge of structural biology. For
traditional crystallography experiments, the growth of crystals with large size and high …

[HTML][HTML] The single particles, clusters and biomolecules and serial femtosecond crystallography instrument of the European XFEL: initial installation

AP Mancuso, A Aquila, L Batchelor, RJ Bean… - Synchrotron …, 2019 - journals.iucr.org
The European X-ray Free-Electron Laser (FEL) became the first operational high-repetition-
rate hard X-ray FEL with first lasing in May 2017. Biological structure determination has …

Structural mass spectrometry goes viral

J Duelfer, A Kadek, JD Kopicki, B Krichel… - Advances in virus …, 2019 - Elsevier
Over the last 20 years, mass spectrometry (MS), with its ability to analyze small sample
amounts with high speed and sensitivity, has more and more entered the field of structural …

Megahertz single-particle imaging at the European XFEL

E Sobolev, S Zolotarev, K Giewekemeyer… - Communications …, 2020 - nature.com
The emergence of high repetition-rate X-ray free-electron lasers (XFELs) powered by
superconducting accelerator technology enables the measurement of significantly more …

Helium-electrospray improves sample delivery in X-ray single-particle imaging experiments

TV Yenupuri, S Rafie-Zinedine, L Worbs… - Scientific Reports, 2024 - nature.com
Imaging the structure and observing the dynamics of isolated proteins using single-particle X-
ray diffractive imaging (SPI) is one of the potential applications of X-ray free-electron lasers …

3D diffractive imaging of nanoparticle ensembles using an x-ray laser

K Ayyer, PL Xavier, J Bielecki, Z Shen, BJ Daurer… - Optica, 2020 - opg.optica.org
Single particle imaging at x-ray free electron lasers (XFELs) has the potential to determine
the structure and dynamics of single biomolecules at room temperature. Two major hurdles …

Three-dimensional coherent x-ray diffraction imaging via deep convolutional neural networks

L Wu, S Yoo, AF Suzana, TA Assefa, J Diao… - npj Computational …, 2021 - nature.com
As a critical component of coherent X-ray diffraction imaging (CDI), phase retrieval has been
extensively applied in X-ray structural science to recover the 3D morphological information …

Complex imaging of phase domains by deep neural networks

L Wu, P Juhas, S Yoo, I Robinson - IUCrJ, 2021 - journals.iucr.org
The reconstruction of a single-particle image from the modulus of its Fourier transform, by
phase-retrieval methods, has been extensively applied in X-ray structural science …

[HTML][HTML] Perspectives on single particle imaging with x rays at the advent of high repetition rate x-ray free electron laser sources

J Bielecki, FRNC Maia, AP Mancuso - Structural Dynamics, 2020 - pubs.aip.org
X-ray free electron lasers (XFELs) now routinely produce millijoule level pulses of x-ray
photons with tens of femtoseconds duration. Such x-ray intensities gave rise to the idea that …

Low-signal limit of X-ray single particle diffractive imaging

K Ayyer, AJ Morgan, A Aquila, H DeMirci, BG Hogue… - Optics express, 2019 - opg.optica.org
An outstanding question in X-ray single particle imaging experiments has been the
feasibility of imaging sub 10-nm-sized biomolecules under realistic experimental conditions …