The emerging role of mass spectrometry-based proteomics in drug discovery
Proteins are the main targets of most drugs; however, system-wide methods to monitor
protein activity and function are still underused in drug discovery. Novel biochemical …
protein activity and function are still underused in drug discovery. Novel biochemical …
Mass spectrometry‐based high‐throughput proteomics and its role in biomedical studies and systems biology
There are multiple reasons why the next generation of biological and medical studies
require increasing numbers of samples. Biological systems are dynamic, and the effect of a …
require increasing numbers of samples. Biological systems are dynamic, and the effect of a …
A proteome-wide atlas of drug mechanism of action
Defining the cellular response to pharmacological agents is critical for understanding the
mechanism of action of small molecule perturbagens. Here, we developed a 96-well-plate …
mechanism of action of small molecule perturbagens. Here, we developed a 96-well-plate …
Proteome integral solubility alteration: a high-throughput proteomics assay for target deconvolution
Various agents, including drugs as well as nonmolecular stimuli, induce alterations in the
physicochemical properties of proteins in cell lysates, living cells, and organisms. These …
physicochemical properties of proteins in cell lysates, living cells, and organisms. These …
Targeting ribosome biogenesis in cancer: lessons learned and way forward
A Zisi, J Bartek, MS Lindström - Cancers, 2022 - mdpi.com
Simple Summary Cells need to produce ribosomes to sustain continuous proliferation and
expand in numbers, a feature that is even more prominent in uncontrollably proliferating …
expand in numbers, a feature that is even more prominent in uncontrollably proliferating …
Alternative RNA splicing modulates ribosomal composition and determines the spatial phenotype of glioblastoma cells
TD Larionova, S Bastola, TE Aksinina, KS Anufrieva… - Nature cell …, 2022 - nature.com
Glioblastoma (GBM) is characterized by exceptionally high intratumoral heterogeneity.
However, the molecular mechanisms underlying the origin of different GBM cell populations …
However, the molecular mechanisms underlying the origin of different GBM cell populations …
Computational analyses of mechanism of action (MoA): data, methods and integration
The elucidation of a compound's Mechanism of Action (MoA) is a challenging task in the
drug discovery process, but it is important in order to rationalise phenotypic findings and to …
drug discovery process, but it is important in order to rationalise phenotypic findings and to …
Deep proteomic compound profiling with the orbitrap ascend tribrid mass spectrometer using tandem mass tags and real-time search
Tandem mass tags (TMT) and tribrid mass spectrometers are a powerful combination for
high-throughput proteomics with high quantitative accuracy. Increasingly, this technology is …
high-throughput proteomics with high quantitative accuracy. Increasingly, this technology is …
[HTML][HTML] Comprehensive chemical proteomics for target deconvolution of the redox active drug auranofin
Chemical proteomics encompasses novel drug target deconvolution methods in which
compound modification is not required. Herein we use Thermal Proteome Profiling …
compound modification is not required. Herein we use Thermal Proteome Profiling …
[HTML][HTML] System-wide identification and prioritization of enzyme substrates by thermal analysis
Despite the immense importance of enzyme–substrate reactions, there is a lack of general
and unbiased tools for identifying and prioritizing substrate proteins that are modified by the …
and unbiased tools for identifying and prioritizing substrate proteins that are modified by the …