Budding yeast as a model organism to study the effects of age

A Denoth Lippuner, T Julou… - FEMS microbiology …, 2014 - academic.oup.com
Although a budding yeast culture can be propagated eternally, individual yeast cells age
and eventually die. The detailed knowledge of this unicellular eukaryotic species as well as …

Recent advances in microfluidic devices for single-cell cultivation: methods and applications

D Anggraini, N Ota, Y Shen, T Tang, Y Tanaka… - Lab on a Chip, 2022 - pubs.rsc.org
Single-cell analysis is essential to improve our understanding of cell functionality from
cellular and subcellular aspects for diagnosis and therapy. Single-cell cultivation is one of …

Life span extension by glucose restriction is abrogated by methionine supplementation: Cross-talk between glucose and methionine and implication of methionine as …

K Zou, S Rouskin, K Dervishi, MA McCormick… - Science …, 2020 - science.org
Caloric restriction (CR) is known to extend life span across species; however, the molecular
mechanisms are not well understood. We investigate the mechanism by which glucose …

Protein biogenesis machinery is a driver of replicative aging in yeast

GE Janssens, AC Meinema, J Gonzalez, JC Wolters… - elife, 2015 - elifesciences.org
An integrated account of the molecular changes occurring during the process of cellular
aging is crucial towards understanding the underlying mechanisms. Here, using novel …

High-throughput analysis of yeast replicative aging using a microfluidic system

MC Jo, W Liu, L Gu, W Dang… - Proceedings of the …, 2015 - National Acad Sciences
Saccharomyces cerevisiae has been an important model for studying the molecular
mechanisms of aging in eukaryotic cells. However, the laborious and low-throughput …

A microfluidic system for studying ageing and dynamic single-cell responses in budding yeast

MM Crane, IBN Clark, E Bakker, S Smith, PS Swain - PloS one, 2014 - journals.plos.org
Recognition of the importance of cell-to-cell variability in cellular decision-making and a
growing interest in stochastic modeling of cellular processes has led to an increased …

Age-dependent deterioration of nuclear pore assembly in mitotic cells decreases transport dynamics

IL Rempel, MM Crane, DJ Thaller, A Mishra… - Elife, 2019 - elifesciences.org
Nuclear transport is facilitated by the Nuclear Pore Complex (NPC) and is essential for life in
eukaryotes. The NPC is a long-lived and exceptionally large structure. We asked whether …

Early telomerase inactivation accelerates aging independently of telomere length

Z **e, KA Jay, DL Smith, Y Zhang, Z Liu, J Zheng… - Cell, 2015 - cell.com
Telomerase is required for long-term telomere maintenance and protection. Using single
budding yeast mother cell analyses we found that, even early after telomerase inactivation …

[HTML][HTML] Aging yeast cells undergo a sharp entry into senescence unrelated to the loss of mitochondrial membrane potential

S Fehrmann, C Paoletti, Y Goulev, A Ungureanu… - Cell reports, 2013 - cell.com
In budding yeast, a mother cell can produce a finite number of daughter cells before it stops
dividing and dies. Such entry into senescence is thought to result from a progressive decline …

A new experimental platform facilitates assessment of the transcriptional and chromatin landscapes of aging yeast

DG Hendrickson, I Soifer, BJ Wranik, G Kim, M Robles… - elife, 2018 - elifesciences.org
Replicative aging of Saccharomyces cerevisiae is an established model system for
eukaryotic cellular aging. A limitation in yeast lifespan studies has been the difficulty of …