Towards a seamless product and process development workflow for recombinant proteins produced by plant molecular farming

JF Buyel - Biotechnology Advances, 2024 - Elsevier
Plant molecular farming (PMF) has been promoted as a fast, efficient and cost-effective
alternative to bacteria and animal cells for the production of biopharmaceutical proteins …

Advances in subcellular accumulation design for recombinant protein production in tobacco

SJ Song, HP Diao, YF Guo, I Hwang - BioDesign Research, 2024 - spj.science.org
Plants and their use as bioreactors for the generation of recombinant proteins have become
one of the hottest topics in the field of Plant Biotechnology and Plant Synthetic Biology. Plant …

Wheat zinc finger protein TaZF interacts with both the powdery mildew AvrPm2 protein and the corresponding wheat Pm2a immune receptor

B Manser, H Zbinden, G Herren, J Steger… - Plant …, 2024 - cell.com
Plant defense responses to pathogens are induced after direct or indirect perception of
effector proteins or their activity on host proteins. In fungal–plant interactions, relatively little …

RPG acts as a central determinant for infectosome formation and cellular polarization during intracellular rhizobial infections

B Lace, C Su, DI Perez, M Rodriguez-Franco, T Vernié… - Elife, 2023 - elifesciences.org
Host-controlled intracellular accommodation of nitrogen-fixing bacteria is essential for the
establishment of a functional Root Nodule Symbiosis (RNS). In many host plants, this occurs …

Binary vector copy number engineering improves Agrobacterium-mediated transformation

MJ Szarzanowicz, LM Waldburger, M Busche… - Nature …, 2024 - nature.com
The copy number of a plasmid is linked to its functionality, yet there have been few attempts
to optimize higher-copy-number mutants for use across diverse origins of replication in …

In vivo biosensing of subcellular pyruvate pools reveals photosynthesis-dependent metabolite dynamics in Nicotiana benthamiana

J Multhoff, JO Niemeier, K Zheng… - Journal of …, 2024 - academic.oup.com
Pyruvate is central to metabolism across biology. It acts as a metabolic hub linking major
pathways including glycolysis, the Krebs cycle, fermentation, and synthesis of amino acids …

An AGO10: miR165/6 module regulates meristem activity and xylem development in the Arabidopsis root

S Mirlohi, G Schott, A Imboden, O Voinnet - The EMBO Journal, 2024 - embopress.org
The RNA-silencing effector ARGONAUTE10 influences cell fate in plant shoot and floral
meristems. ARGONAUTE10 also accumulates in the root apical meristem (RAM), yet its …

Metabolic engineering of omega-3 long chain polyunsaturated fatty acids in plants using different∆ 6-and∆ 5-desaturases co-expressed with LPCAT from the marine …

S Klińska-Bąchor, K Demski, Y Gong, A Banaś - Scientific Reports, 2024 - nature.com
Continuous research on obtaining an even more efficient production of very long-chain
polyunsaturated fatty acids (VLC-PUFAs) in plants remains one of the main challenges of …

Genetically clustered antifungal phytocytokines and receptor protein family members cooperate to trigger plant immune signaling

J Lintz, Y Goto, KW Bender, R Bchini… - Journal of …, 2024 - academic.oup.com
Phytocytokines regulate plant immunity by cooperating with cell surface proteins. Populus
trichocarpa RUST INDUCED SECRETED PEPTIDE 1 (PtRISP1) exhibits an elicitor activity in …

Arabidopsis VQ motif-containing proteins VQ1 and VQ10 interact with plastidial 1-deoxy-D-xylulose-5-phosphate synthase

B Gayubas, MC Castillo, J León - Scientific Reports, 2024 - nature.com
VQ1 and VQ10 are largely unstructured homologous proteins with a significant potential for
protein–protein interactions. Yeast two-hybrid (Y2H) analysis confirmed that both proteins …