Variant calling and benchmarking in an era of complete human genome sequences

ND Olson, J Wagner, N Dwarshuis, KH Miga… - Nature Reviews …, 2023 - nature.com
Genetic variant calling from DNA sequencing has enabled understanding of germline
variation in hundreds of thousands of humans. Sequencing technologies and variant-calling …

eXclusionarY: 10 years later, where are the sex chromosomes in GWASs?

L Sun, Z Wang, T Lu, TA Manolio… - The American Journal of …, 2023 - cell.com
Summary 10 years ago, a detailed analysis showed that only 33% of genome-wide
association study (GWAS) results included the X chromosome. Multiple recommendations …

Telomere-to-telomere assembly of diploid chromosomes with Verkko

M Rautiainen, S Nurk, BP Walenz, GA Logsdon… - Nature …, 2023 - nature.com
Abstract The Telomere-to-Telomere consortium recently assembled the first truly complete
sequence of a human genome. To resolve the most complex repeats, this project relied on …

Assembly of 43 human Y chromosomes reveals extensive complexity and variation

P Hallast, P Ebert, M Loftus, F Yilmaz, PA Audano… - Nature, 2023 - nature.com
The prevalence of highly repetitive sequences within the human Y chromosome has
prevented its complete assembly to date and led to its systematic omission from genomic …

The complete and fully-phased diploid genome of a male Han Chinese

C Yang, Y Zhou, Y Song, D Wu, Y Zeng, L Nie, P Liu… - Cell Research, 2023 - nature.com
Since the release of the complete human genome, the priority of human genomic study has
now been shifting towards closing gaps in ethnic diversity. Here, we present a fully phased …

Gapless assembly of complete human and plant chromosomes using only nanopore sequencing

S Koren, Z Bao, A Guarracino, S Ou… - Genome …, 2024 - genome.cshlp.org
The combination of ultra-long (UL) Oxford Nanopore Technologies (ONT) sequencing reads
with long, accurate Pacific Bioscience (PacBio) High Fidelity (HiFi) reads has enabled the …

Recombination between heterologous human acrocentric chromosomes

A Guarracino, S Buonaiuto, LG de Lima, T Potapova… - Nature, 2023 - nature.com
The short arms of the human acrocentric chromosomes 13, 14, 15, 21 and 22 (SAACs) share
large homologous regions, including ribosomal DNA repeats and extended segmental …

The complete sequence and comparative analysis of ape sex chromosomes

KD Makova, BD Pickett, RS Harris, GA Hartley… - Nature, 2024 - nature.com
Apes possess two sex chromosomes—the male-specific Y chromosome and the X
chromosome, which is present in both males and females. The Y chromosome is crucial for …

T2T-YAO: a telomere-to-telomere assembled diploid reference genome for Han Chinese

Y He, Y Chu, S Guo, J Hu, R Li, Y Zheng… - Genomics …, 2023 - academic.oup.com
Since its initial release in 2001, the human reference genome has undergone continuous
improvement in quality, and the recently released telomere-to-telomere (T2T) version—T2T …

Scalable, accessible and reproducible reference genome assembly and evaluation in Galaxy

D Larivière, L Abueg, N Brajuka, C Gallardo-Alba… - Nature …, 2024 - nature.com
The Earth BioGenome Project aims to produce reference genomes for all~ 1.8 million known
eukaryotic species over the next decade 1–4. Achieving this goal will require the current …