Solid-state NMR: Methods for biological solids
In the last two decades, solid-state nuclear magnetic resonance (ssNMR) spectroscopy has
transformed from a spectroscopic technique investigating small molecules and industrial …
transformed from a spectroscopic technique investigating small molecules and industrial …
Multiplexing experiments in NMR and multi-nuclear MRI
Multiplexing NMR experiments by direct detection of multiple free induction decays (FIDs) in
a single experiment offers a dramatic increase in the spectral information content and often …
a single experiment offers a dramatic increase in the spectral information content and often …
CAPITO—a web server-based analysis and plotting tool for circular dichroism data
C Wiedemann, P Bellstedt, M Görlach - Bioinformatics, 2013 - academic.oup.com
Motivation: Circular dichroism (CD) spectroscopy is one of the most versatile tools to study
protein folding and to validate the proper fold of purified proteins. Here, we aim to provide a …
protein folding and to validate the proper fold of purified proteins. Here, we aim to provide a …
Sequential backbone assignment based on dipolar amide-to-amide correlation experiments
SQ **ang, K Grohe, P Rovó, SK Vasa, K Giller… - Journal of biomolecular …, 2015 - Springer
Proton detection in solid-state NMR has seen a tremendous increase in popularity in the last
years. New experimental techniques allow to exploit protons as an additional source of …
years. New experimental techniques allow to exploit protons as an additional source of …
PHRONESIS: A One‐Shot Approach for Sequential Assignment of Protein Resonances by Ultrafast MAS Solid‐State NMR Spectroscopy
Solid‐state NMR (ssNMR) spectroscopy has emerged as the method of choice to analyze
the structural dynamics of fibrillar, membrane‐bound, and crystalline proteins that are …
the structural dynamics of fibrillar, membrane‐bound, and crystalline proteins that are …
Multi-receiver solid-state NMR using polarization optimized experiments (POE) at ultrafast magic angle spinning
Ultrafast magic angle spinning (MAS) technology and 1 H detection have dramatically
enhanced the sensitivity of solid-state NMR (ssNMR) spectroscopy of biopolymers. We …
enhanced the sensitivity of solid-state NMR (ssNMR) spectroscopy of biopolymers. We …
Orphan spin operators enable the acquisition of multiple 2D and 3D magic angle spinning solid-state NMR spectra
We propose a general method that enables the acquisition of multiple 2D and 3D solid-state
NMR spectra for U-13 C, 15 N-labeled proteins. This method, called MEIOSIS (Multiple …
NMR spectra for U-13 C, 15 N-labeled proteins. This method, called MEIOSIS (Multiple …
A suite of pulse sequences based on multiple sequential acquisitions at one and two radiofrequency channels for solid-state magic-angle spinning NMR studies of …
One of the fundamental challenges in the application of solid-state NMR is its limited
sensitivity, yet a majority of experiments do not make efficient use of the limited polarization …
sensitivity, yet a majority of experiments do not make efficient use of the limited polarization …
Proton-detected polarization optimized experiments (POE) using ultrafast magic angle spinning solid-state NMR: Multi-acquisition of membrane protein spectra
Proton-detected solid-state NMR (ssNMR) spectroscopy has dramatically improved the
sensitivity and resolution of fast magic angle spinning (MAS) methods. While relatively …
sensitivity and resolution of fast magic angle spinning (MAS) methods. While relatively …
Access to side-chain carbon information in deuterated solids under fast MAS through non-rotor-synchronized mixing
N Kulminskaya, SK Vasa, K Giller, S Becker… - Chemical …, 2016 - pubs.rsc.org
We demonstrate the accessibility of aliphatic 13C side chain chemical shift sets for solid-
state NMR despite perdeuteration and fast MAS using isotropic, non-rotor-synchronized 13C …
state NMR despite perdeuteration and fast MAS using isotropic, non-rotor-synchronized 13C …