Heparan sulfate regulates IL-21 bioavailability and signal strength that control germinal center B cell selection and differentiation

Z Chen, Y Cui, Y Yao, B Liu, J Yunis, X Gao… - Science …, 2023 - science.org
In antibody responses, mutated germinal center B (BGC) cells are positively selected for
reentry or differentiation. As the products from GCs, memory B cells and antibody-secreting …

Protein purification strategies must consider downstream applications and individual biological characteristics

K Remans, M Lebendiker, C Abreu, M Maffei… - Microbial Cell …, 2022 - Springer
Background Proteins are used as reagents in a broad range of scientific fields. The reliability
and reproducibility of experimental data will largely depend on the quality of the …

[HTML][HTML] Recombinant N-terminal outer membrane porin (OprF) of Pseudomonas aeruginosa is a promising vaccine candidate against both P. aeruginosa and some …

M Bahey-El-Din, SA Mohamed, SA Sheweita… - International Journal of …, 2020 - Elsevier
Pseudomonas aeruginosa is an evolving pathogen which can cause serious infections
especially to immunocompromised patients. Its high resistance profile to antibiotics results in …

Immunization with the ferric iron-binding periplasmic protein HitA provides protection against Pseudomonas aeruginosa in the murine infection model

MA Elhosary, M Bahey-El-Din, A AbdelBary… - Microbial …, 2019 - Elsevier
Pseudomonas aeruginosa is a notorious pathogen with increasing multi-drug resistance.
This situation makes it urgent to develop a prophylactic vaccine against this pathogen …

High-level expression and efficient refolding of therapeutically important recombinant human Interleukin-3 (hIL-3) in E. coli

VK Dagar, YP Khasa - Protein Expression and Purification, 2017 - Elsevier
Abstract Human interleukin-3 (hIL-3) is a pleiotropic cytokine that stimulates the
differentiation and proliferation of multipotent hematopoietic cells thus making it a …

A chemokine-fusion vaccine targeting immature dendritic cells elicits elevated antibody responses to malaria sporozoites in infant macaques

K Luo, JT Gordy, F Zavala, RB Markham - Scientific reports, 2021 - nature.com
Infants and young children are the groups at greatest risk for severe disease resulting from
Plasmodium falciparum infection. We previously demonstrated in mice that a protein vaccine …

Recombinant Ax21 protein is a promising subunit vaccine candidate against Stenotrophomonas maltophilia in a murine infection model

AT Sarhan, M Bahey-El-Din, TI Zaghloul - Vaccine, 2021 - Elsevier
Stenotrophomonas maltophilia is an emerging pathogen that can cause several disease
manifestations such as bacteremia, meningitis, respiratory tract infections and others. More …

Potent antitumor activity of a designed interleukin-21 mimic

JH Chun, BS Lim, S Roy, MJ Walsh, GC Abhiraman… - bioRxiv, 2024 - biorxiv.org
Long-standing goals of cancer immunotherapy are to activate cytotoxic antitumor T cells
across a broad range of affinities while dampening suppressive regulatory T (Treg) cell …

Extended protection capabilities of an immature dendritic-cell targeting malaria sporozoite vaccine

K Luo, F Zavala, J Gordy, H Zhang, RB Markham - Vaccine, 2017 - Elsevier
Mouse studies evaluating candidate malaria vaccines have typically examined protective
efficacy over the relatively short time frames of several weeks after the final of multiple …

[HTML][HTML] Immunization with the basic membrane protein (BMP) family ABC transporter elicits protection against Enterococcus faecium in a murine infection model

SW Yousief, M Bahey-El-Din, TI Zaghloul - Microbes and Infection, 2020 - Elsevier
Enterococcus faecium is evolving as a multi-resistant pathogen causing infections with high
morbidity and mortality. A protective vaccine against E. faecium is lacking up till now. ATP …