A third of the genes in prokaryotic and eukaryotic genomes encode membrane proteins that
are either essential for signal transduction and solute transport or function as scaffold …

This review focuses upon the application of solution NMR methods to multispan integral
membrane proteins, particularly with respect to determination of global folds by this …

Membrane proteins are encoded by 20–35% of genes but represent< 1% of known protein
structures to date. Thus, improved methods for membrane-protein structure determination …

Contraction and relaxation of heart muscle cells is regulated by cycling of calcium between
cytoplasm and sarcoplasmic reticulum. Human phospholamban (PLN), expressed in the …

Escherichia coli diacylglycerol kinase (DAGK) represents a family of integral membrane
enzymes that is unrelated to all other phosphotransferases. We have determined the three …

Determination of structures and dynamics events of transmembrane proteins is important for
the understanding of their function. Analysis of such events requires high-resolution 3D …

We have compared four different vectors for expression of proteins with N-or C-terminal
hexahistidine (His 6) tags in Escherichia coli by testing these on 20 human proteins. We …

A major challenge for the structure determination of integral membrane proteins by solution
NMR spectroscopy is the limited number of NOE restraints in these systems stemming from …

Protein crystallography is used to generate atomic resolution structures of protein molecules.
These structures provide information about biological function, mechanism and interaction of …

Nuclear magnetic resonance (NMR) studies of large membrane‐associated proteins are
limited by the difficulties in preparation of stable protein–detergent mixed micelles and by …