Plant genome sequence assembly in the era of long reads: Progress, challenges and future directions

B Pucker, I Irisarri, J de Vries, B Xu - Quantitative Plant Biology, 2022 - cambridge.org
Third-generation long-read sequencing is transforming plant genomics. Oxford Nanopore
Technologies and Pacific Biosciences are offering competing long-read sequencing …

Towards complete and error-free genome assemblies of all vertebrate species

A Rhie, SA McCarthy, O Fedrigo, J Damas, G Formenti… - Nature, 2021 - nature.com
High-quality and complete reference genome assemblies are fundamental for the
application of genomics to biology, disease, and biodiversity conservation. However, such …

Tools and strategies for long-read sequencing and de novo assembly of plant genomes

H Jung, C Winefield, A Bombarely, P Prentis… - Trends in plant …, 2019 - cell.com
The commercial release of third-generation sequencing technologies (TGSTs), giving long
and ultra-long sequencing reads, has stimulated the development of new tools for …

Complete vertebrate mitogenomes reveal widespread repeats and gene duplications

G Formenti, A Rhie, J Balacco, B Haase, J Mountcastle… - Genome biology, 2021 - Springer
Background Modern sequencing technologies should make the assembly of the relatively
small mitochondrial genomes an easy undertaking. However, few tools exist that address …

Plastid genome assembly using long‐read data

W Zhou, CE Armijos, C Lee, R Lu… - Molecular Ecology …, 2023 - Wiley Online Library
Although plastid genome (plastome) structure is highly conserved across most seed plants,
investigations during the past two decades have revealed several disparately related …

Two long read-based genome assembly and annotation of polyploidy woody plants, Hibiscus syriacus L. using PacBio and Nanopore platforms

H Koo, GW Lee, SR Ko, S Go, SY Kwon, YM Kim… - Scientific Data, 2023 - nature.com
Improvements in long read DNA sequencing and related techniques facilitated the
generation of complex eukaryotic genomes. Despite these advances, the quality of …

Complete chloroplast genome of Lilium ledebourii (Baker) Boiss and its comparative analysis: lights into selective pressure and adaptive evolution

M Sheikh-Assadi, R Naderi, M Kafi, R Fatahi… - Scientific Reports, 2022 - nature.com
Abstract Lilium ledebourii (Baker) Boiss is a rare species, which exhibits valuable traits.
However, before its genetic diversity and evolutionary were uncovered, its wild resources …

A systematic comparison of chloroplast genome assembly tools

JA Freudenthal, S Pfaff, N Terhoeven, A Korte… - Genome Biology, 2020 - Springer
Background Chloroplasts are intracellular organelles that enable plants to conduct
photosynthesis. They arose through the symbiotic integration of a prokaryotic cell into an …

Chlorella vulgaris genome assembly and annotation reveals the molecular basis for metabolic acclimation to high light conditions

M Cecchin, L Marcolungo, M Rossato… - The Plant …, 2019 - Wiley Online Library
Chlorella vulgaris is a fast‐growing fresh‐water microalga cultivated on the industrial scale
for applications ranging from food to biofuel production. To advance our understanding of its …

[PDF][PDF] TIPPo: A User-Friendly Tool for De Novo Assembly of Organellar Genomes with High-Fidelity Data

W **an, I Bezrukov, Z Bao, S Vorbrugg… - Molecular Biology …, 2025 - academic.oup.com
Plant cells have two major organelles with their own genomes: chloroplasts and
mitochondria. While chloroplast genomes tend to be structurally conserved, the …