Design and application of fluorescent probes to detect cellular physical microenvironments

J Ma, R Sun, K **a, Q **a, Y Liu, X Zhang - Chemical Reviews, 2024 - ACS Publications
The microenvironment is indispensable for functionality of various biomacromolecules,
subcellular compartments, living cells, and organisms. In particular, physical properties …

Fluorescence lifetime imaging microscopy: fundamentals and advances in instrumentation, analysis, and applications

R Datta, TM Heaster, JT Sharick… - … of biomedical optics, 2020 - spiedigitallibrary.org
Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Luminescence based temperature bio-imaging: Status, challenges, and perspectives

A Bednarkiewicz, J Drabik, K Trejgis, D Jaque… - Applied Physics …, 2021 - pubs.aip.org
The only way to get thermal images of living organisms without perturbing them is to use
luminescent probes with temperature-dependent spectral properties. The acquisition of such …

Carbon dot nanothermometry: intracellular photoluminescence lifetime thermal sensing

S Kalytchuk, K Poláková, Y Wang, JP Froning… - ACS …, 2017 - ACS Publications
Nanoscale biocompatible photoluminescence (PL) thermometers that can be used to
accurately and reliably monitor intracellular temperatures have many potential applications …

Optical nanoscale thermometry: from fundamental mechanisms to emerging practical applications

C Bradac, SF Lim, HC Chang… - Advanced Optical …, 2020 - Wiley Online Library
Abstract Knowledge of temperature and temperature gradients with nanoscale resolution is
critical for a variety of applications in medicine, nanoelectronics, biology, and solid‐state …

Micro/nanoscale thermometry for cellular thermal sensing

T Bai, N Gu - Small, 2016 - Wiley Online Library
Temperature is a key parameter to regulate cell function, and biochemical reactions inside a
cell in turn affect the intracellular temperature. It's vitally necessary to measure cellular …

Accurate in vivo nanothermometry through NIR‐II lanthanide luminescence lifetime

M Tan, F Li, N Cao, H Li, X Wang, C Zhang, D Jaque… - Small, 2020 - Wiley Online Library
Luminescence nanothermometry is promising for noninvasive probing of temperature in
biological microenvironment at nanometric spatial resolution. Yet, wavelength‐and …

Intracellular temperature measurements with fluorescent polymeric thermometers

S Uchiyama, C Gota, T Tsuji, N Inada - Chemical Communications, 2017 - pubs.rsc.org
In 2003, we successfully created the first fluorescent polymeric thermometer by combining a
thermo-responsive polymer and an environment-sensitive (polarity and hydrogen bonding …

Temperature imaging using a cationic linear fluorescent polymeric thermometer and fluorescence lifetime imaging microscopy

N Inada, N Fukuda, T Hayashi, S Uchiyama - Nature protocols, 2019 - nature.com
Temperature is one of the most important of the physiological parameters that determine the
biological status of living organisms. However, intracellular temperature was not imaged at …

Mitochondrial temperature homeostasis resists external metabolic stresses

M Terzioglu, K Veeroja, T Montonen, TO Ihalainen… - Elife, 2023 - elifesciences.org
Based on studies with a fluorescent reporter dye, Mito Thermo Yellow (MTY), and the
genetically encoded gTEMP ratiometric fluorescent temperature indicator targeted to …