Performing skin microbiome research: a method to the madness
Growing interest in microbial contributions to human health and disease has increasingly
led investigators to examine the microbiome in both healthy skin and cutaneous disorders …
led investigators to examine the microbiome in both healthy skin and cutaneous disorders …
Metagenomic assembly through the lens of validation: recent advances in assessing and improving the quality of genomes assembled from metagenomes
Metagenomic samples are snapshots of complex ecosystems at work. They comprise
hundreds of known and unknown species, contain multiple strain variants and vary greatly …
hundreds of known and unknown species, contain multiple strain variants and vary greatly …
[KSIĄŻKA][B] A practical guide to DNA-based methods for biodiversity assessment.
This book represents a synthesis of knowledge and best practice in the field of DNA-based
biomonitoring at the time of writing. It has been written with end-users of molecular tools in …
biomonitoring at the time of writing. It has been written with end-users of molecular tools in …
Impact of DNA extraction method and targeted 16S-rRNA hypervariable region on oral microbiota profiling
Amplification and sequencing of 16S amplicons are widely used for profiling the structure of
oral microbiota. However, it remains not clear whether and to what degree DNA extraction …
oral microbiota. However, it remains not clear whether and to what degree DNA extraction …
Effect of DNA extraction methods and sampling techniques on the apparent structure of cow and sheep rumen microbial communities
Molecular microbial ecology techniques are widely used to study the composition of the
rumen microbiota and to increase understanding of the roles they play. Therefore, sampling …
rumen microbiota and to increase understanding of the roles they play. Therefore, sampling …
Evaluating the impact of DNA extraction method on the representation of human oral bacterial and fungal communities
The application of high-throughput, next-generation sequencing technologies has greatly
improved our understanding of the human oral microbiome. While deciphering this diverse …
improved our understanding of the human oral microbiome. While deciphering this diverse …
Quantification of DNA through the NanoDrop spectrophotometer: methodological validation using standard reference material and Sprague Dawley rat and human …
AM García-Alegría, I Anduro-Corona… - … journal of analytical …, 2020 - Wiley Online Library
This study aimed to validate an analytical method to determine DNA concentration using
standard reference material (NIST SRM 2372) and Sprague Dawley rat and human DNA …
standard reference material (NIST SRM 2372) and Sprague Dawley rat and human DNA …
[HTML][HTML] Mu-DNA: a modular universal DNA extraction method adaptable for a wide range of sample types
Efficient DNA extraction is fundamental to molecular studies. However, commercial kits are
expensive when a large number of samples need to be processed. Here we present a …
expensive when a large number of samples need to be processed. Here we present a …
Analysis and correction of compositional bias in sparse sequencing count data
Background Count data derived from high-throughput deoxy-ribonucliec acid (DNA)
sequencing is frequently used in quantitative molecular assays. Due to properties inherent …
sequencing is frequently used in quantitative molecular assays. Due to properties inherent …
Benchmarking DNA isolation methods for marine metagenomics
A Demkina, D Slonova, V Mamontov, O Konovalova… - Scientific reports, 2023 - nature.com
Metagenomics is a powerful tool to study marine microbial communities. However, obtaining
high-quality environmental DNA suitable for downstream sequencing applications is a …
high-quality environmental DNA suitable for downstream sequencing applications is a …