Recent developments in bioprocessing of recombinant proteins: expression hosts and process development
NK Tripathi, A Shrivastava - Frontiers in Bioengineering and …, 2019 - frontiersin.org
Infectious diseases, along with cancers, are among the main causes of death among
humans worldwide. The production of therapeutic proteins for treating diseases at large …
humans worldwide. The production of therapeutic proteins for treating diseases at large …
Application of different types of CRISPR/Cas-based systems in bacteria
Z Liu, H Dong, Y Cui, L Cong, D Zhang - Microbial cell factories, 2020 - Springer
As important genome editing tools, CRISPR/Cas systems, especially those based on type II
Cas9 and type V Cas12a, are widely used in genetic and metabolic engineering of bacteria …
Cas9 and type V Cas12a, are widely used in genetic and metabolic engineering of bacteria …
Escherichia coli as a host for metabolic engineering
Over the past century, Escherichia coli has become one of the best studied organisms on
earth. Features such as genetic tractability, favorable growth conditions, well characterized …
earth. Features such as genetic tractability, favorable growth conditions, well characterized …
CRISPR-Cas9 for the genome engineering of cyanobacteria and succinate production
H Li, CR Shen, CH Huang, LY Sung, MY Wu… - Metabolic …, 2016 - Elsevier
Cyanobacteria hold promise as a cell factory for producing biofuels and bio-derived
chemicals, but genome engineering of cyanobacteria such as Synechococcus elongatus …
chemicals, but genome engineering of cyanobacteria such as Synechococcus elongatus …
Development of a CRISPR/Cas9 genome editing toolbox for Corynebacterium glutamicum
Background Corynebacterium glutamicum is an important industrial workhorse and
advanced genetic engineering tools are urgently demanded. Recently, the clustered …
advanced genetic engineering tools are urgently demanded. Recently, the clustered …
CRISPR/Cas9-based genome editing of the filamentous fungi: the state of the art
TQ Shi, GN Liu, RY Ji, K Shi, P Song, LJ Ren… - Applied microbiology …, 2017 - Springer
In recent years, a variety of genetic tools have been developed and applied to various
filamentous fungi, which are widely applied in agriculture and the food industry. However …
filamentous fungi, which are widely applied in agriculture and the food industry. However …
CRISPR technologies for bacterial systems: current achievements and future directions
Throughout the decades of its history, the advances in bacteria-based bio-industries have
coincided with great leaps in strain engineering technologies. Recently unveiled clustered …
coincided with great leaps in strain engineering technologies. Recently unveiled clustered …
CRISPR interference (CRISPRi) for gene regulation and succinate production in cyanobacterium S. elongatus PCC 7942
CH Huang, CR Shen, H Li, LY Sung, MY Wu… - Microbial cell …, 2016 - Springer
Abstract Background Cyanobacterium Synechococcus elongatus PCC 7942 holds promise
for biochemical conversion, but gene deletion in PCC 7942 is time-consuming and may be …
for biochemical conversion, but gene deletion in PCC 7942 is time-consuming and may be …
Repurposing CRISPR RNA-guided integrases system for one-step, efficient genomic integration of ultra-long DNA sequences
Genomic integration techniques offer opportunities for generation of engineered
microorganisms with improved or even entirely new functions but are currently limited by …
microorganisms with improved or even entirely new functions but are currently limited by …
CRISPR technologies for stem cell engineering and regenerative medicine
CRISPR/Cas9 system exploits the concerted action of Cas9 nuclease and programmable
single guide RNA (sgRNA), and has been widely used for genome editing. The Cas9 …
single guide RNA (sgRNA), and has been widely used for genome editing. The Cas9 …