Challenges and triumphs in cryo-electron tomography

RK Hylton, MT Swulius - Iscience, 2021 - cell.com
Cryo-electron tomography has stepped fully into the spotlight. Enthusiasm is high.
Fortunately for us, this is an exciting time to be a cryotomographer, but there is still a way to …

An image processing pipeline for electron cryo‐tomography in RELION‐5

A Burt, B Toader, R Warshamanage… - FEBS Open …, 2024 - Wiley Online Library
Electron tomography of frozen, hydrated samples allows structure determination of
macromolecular complexes that are embedded in complex environments. Provided that the …

Towards full-stack deep learning-empowered data processing pipeline for synchrotron tomography experiments

Z Zhang, C Li, W Wang, Z Dong, G Liu, Y Dong… - The Innovation, 2024 - cell.com
Synchrotron tomography experiments are transitioning into multifunctional, cross-scale, and
dynamic characterizations, enabled by new-generation synchrotron light sources and fast …

Limited view tomographic reconstruction using a cascaded residual dense spatial-channel attention network with projection data fidelity layer

B Zhou, SK Zhou, JS Duncan… - IEEE transactions on …, 2021 - ieeexplore.ieee.org
Limited view tomographic reconstruction aims to reconstruct a tomographic image from a
limited number of projection views arising from sparse view or limited angle acquisitions that …

A closer look at few-shot video classification: A new baseline and benchmark

Z Zhu, L Wang, S Guo, G Wu - arxiv preprint arxiv:2110.12358, 2021 - arxiv.org
The existing few-shot video classification methods often employ a meta-learning paradigm
by designing customized temporal alignment module for similarity calculation. While …

Computational methods for in situ structural studies with cryogenic electron tomography

C Zhao, D Lu, Q Zhao, C Ren, H Zhang… - Frontiers in Cellular …, 2023 - frontiersin.org
Cryo-electron tomography (cryo-ET) plays a critical role in imaging microorganisms in situ in
terms of further analyzing the working mechanisms of viruses and drug exploitation, among …

paghetti Tracer: A Framework for Tracing Semiregular Filamentous Densities in 3D Tomograms

S Sazzed, P Scheible, J He, W Wriggers - Biomolecules, 2022 - mdpi.com
Within cells, cytoskeletal filaments are often arranged into loosely aligned bundles. These
fibrous bundles are dense enough to exhibit a certain regularity and mean direction …

Cryosam: Training-free cryoet tomogram segmentation with foundation models

Y Zhao, H Bian, M Mu, MR Uddin, Z Li, X Li… - … Conference on Medical …, 2024 - Springer
Abstract Cryogenic Electron Tomography (CryoET) is a useful imaging technology in
structural biology that is hindered by its need for manual annotations, especially in particle …

Detecting protein communities in native cell extracts by machine learning: a structural biologist's perspective

FL Kyrilis, J Belapure, PL Kastritis - Frontiers in molecular …, 2021 - frontiersin.org
Native cell extracts hold great promise for understanding the molecular structure of ordered
biological systems at high resolution. This is because higher-order biomolecular …

TLIR: Two-layer iterative refinement model for limited-angle CT reconstruction

Q Li, T Wang, RR Li, Y Qiang, B Zhang, J Sun… - … Signal Processing and …, 2025 - Elsevier
Limited angle reconstruction is a typical ill-posed problem in computed tomography (CT). In
practical applications, due to the limited scanning angles available for fixed scan targets and …