We present a whole-cell fully dynamical kinetic model (WCM) of JCVI-syn3A, a minimal cell
with a reduced genome of 493 genes that has retained few regulatory proteins or small …

Microbiology is traditionally considered within the context of wet laboratory methodologies.
Computational techniques have a great potential to contribute to microbiology. Here, we …

Genomically minimal cells, such as JCVI-syn3. 0 and JCVI-syn3A, offer an empowering
framework to study relationships between genotype and phenotype. With a polygenic basis …

Engineered microorganisms have emerged as viable alternatives for limonene production.
However, issues such as low enzyme abundance or activities, and regulatory …

Cell-free expression (CFE) systems are fundamental to reconstituting metabolic pathways in
vitro toward the construction of a synthetic cell. Although an Escherichia coli-based CFE …

Integrating experiments, theory and simulations into whole-cell models | Nature Methods Skip
to main content Thank you for visiting nature.com. You are using a browser version with limited …

JCVI-syn3A is a genetically minimal bacterial cell, consisting of 493 genes and only a single
543 kbp circular chromosome. Syn3A's genome and physical size are approximately one …

Data from genomics, proteomics, structural biology and cryo-electron microscopy are
integrated into a structural illustration of a cross section through an entire JCVI-syn3. 0 …

The degree to which we can understand the multi-scale organization of cellular life is tied to
how well our models can represent this organization and the processes that drive its …

Living cells display dynamic and complex behaviors. To understand their response and to
infer novel insights not possible with traditional reductionist approaches, over the last few …