Advances in hydrogen/deuterium exchange mass spectrometry and the pursuit of challenging biological systems

EI James, TA Murphree, C Vorauer, JR Engen… - Chemical …, 2021 - ACS Publications
Solution-phase hydrogen/deuterium exchange (HDX) coupled to mass spectrometry (MS) is
a widespread tool for structural analysis across academia and the biopharmaceutical …

[HTML][HTML] Unraveling protein's structural dynamics: from configurational dynamics to ensemble switching guides functional mesoscale assemblies

E Medina, DR Latham, H Sanabria - Current opinion in structural biology, 2021 - Elsevier
Highlights•The unanimated visualization of proteins must be revisited to highlights the
relevance of structural dynamics•A combination of single-molecule multiparameter …

Computational modeling of molecular structures guided by hydrogen-exchange data

D Devaurs, DA Antunes, AJ Borysik - Journal of the American …, 2022 - ACS Publications
Data produced by hydrogen-exchange monitoring experiments have been used in structural
studies of molecules for several decades. Despite uncertainties about the structural …

Integrating single-molecule spectroscopy and simulations for the study of intrinsically disordered proteins

JJ Alston, A Soranno, AS Holehouse - Methods, 2021 - Elsevier
Over the last two decades, intrinsically disordered proteins and protein regions (IDRs) have
emerged from a niche corner of biophysics to be recognized as essential drivers of cellular …

DNA facilitates heterodimerization between human transcription factors FoxP1 and FoxP2 by increasing their conformational flexibility

R Coñuecar, I Asela, M Rivera, P Galaz-Davison… - Iscience, 2023 - cell.com
Transcription factors regulate gene expression by binding to DNA. They have disordered
regions and specific DNA-binding domains. Binding to DNA causes structural changes …

Integrative structural dynamics probing of the conformational heterogeneity in synaptosomal-associated protein 25

N Saikia, IS Yanez-Orozco, R Qiu, P Hao… - Cell Reports Physical …, 2021 - cell.com
Summary SNAP-25 (synaptosomal-associated protein of 25 kDa) is a prototypical
intrinsically disordered protein (IDP) that is unstructured by itself but forms coiled-coil helices …

[HTML][HTML] Domain tethering impacts dimerization and DNA-mediated allostery in the human transcription factor FoxP1

P Cruz, N Paredes, I Asela, N Kolimi… - The Journal of …, 2023 - pubs.aip.org
Transcription factors are multidomain proteins with specific DNA binding and regulatory
domains. In the human FoxP subfamily (FoxP1, FoxP2, FoxP3, and FoxP4) of transcription …

Proteogenomic Approaches to Understand Gene Mutations and Protein Structural Alterations in Colon Cancer

S Sarkar - Physiologia, 2023 - mdpi.com
With colon cancer being one of the deadliest and most common cancers, understanding the
mechanisms behind colon cancer is crucial in improving therapies. One of the newest …

DNA controls the dimerization of the human FoxP1 forkhead domain

N Kolimi, J Ballard, T Peulen, R Goutam… - Cell Reports Physical …, 2024 - cell.com
Transcription factors (TFs) regulate gene expression by binding to specific DNA sequences
and gating access to genes. Even when the binding of TFs and their cofactors to DNA is …

Dissecting the structural and functional consequences of the evolutionary proline–glycine deletion in the wing 1 region of the forkhead domain of human FoxP1

S Tamarín, P Galaz‐Davison… - FEBS …, 2024 - Wiley Online Library
The human FoxP transcription factors dimerize via three‐dimensional domain swap**, a
unique feature among the human Fox family, as result of evolutionary sequence adaptations …