Many bacterial clustered regularly interspaced short palindromic repeats (CRISPR)–
CRISPR-associated (Cas) systems employ the dual RNA–guided DNA endonuclease Cas9 …

Exciting new technologies are often self-limiting in their rollout, as access to state-of-the-art
instrumentation or the need for years of hands-on experience, for better or worse, ensures …

The creation of genome-wide libraries for CRISPR knockout (CRISPRko), interference
(CRISPRi), and activation (CRISPRa) has enabled the systematic interrogation of gene …

Chromatin architecture has been implicated in cell type-specific gene regulatory programs,
yet how chromatin remodels during development remains to be fully elucidated. Here, by …

We recently found that nucleosomes directly block access of CRISPR/Cas9 to DNA. Here,
we build on this observation with a comprehensive algorithm that incorporates chromatin …

Drought episodes decrease plant growth and productivity, which in turn cause high
economic losses. Plants naturally sense and respond to water stress by activating specific …

A large and ever-expanding set of CRISPR–Cas systems now enables the rapid and flexible
manipulation of genomes in both targeted and large-scale experiments. Numerous software …

Determining causal relationships between distinct chromatin features and gene expression,
and ultimately cell behavior, remains a major challenge. Recent developments in targetable …

Generation of functionally mature organs requires exquisite control of transcriptional
programs governing cell state transitions during development. Despite advances in …

Long non-coding RNAs (lncRNAs) represent a huge reservoir of potential cancer targets.
Such" onco-lncRNAs" have resisted traditional RNAi methods, but CRISPR-Cas9 genome …