Third-generation sequencing: the spearhead towards the radical transformation of modern genomics

K Athanasopoulou, MA Boti, PG Adamopoulos… - Life, 2021 - mdpi.com
Although next-generation sequencing (NGS) technology revolutionized sequencing, offering
a tremendous sequencing capacity with groundbreaking depth and accuracy, it continues to …

The evolution, assembly, and dynamics of marine Holobionts

RA González-Pech, VY Li, V Garcia… - Annual Review of …, 2024 - annualreviews.org
The holobiont concept (ie, multiple living beings in close symbiosis with one another and
functioning as a unit) is revolutionizing our understanding of biology, especially in marine …

Identification of nucleoside monophosphates and their epigenetic modifications using an engineered nanopore

Y Wang, S Zhang, W Jia, P Fan, L Wang, X Li… - Nature …, 2022 - nature.com
RNA modifications play critical roles in the regulation of various biological processes and
are associated with many human diseases. Direct identification of RNA modifications by …

[HTML][HTML] Full-length transcript sequencing of human and mouse cerebral cortex identifies widespread isoform diversity and alternative splicing

SK Leung, AR Jeffries, I Castanho, BT Jordan, K Moore… - Cell reports, 2021 - cell.com
Alternative splicing is a post-transcriptional regulatory mechanism producing distinct mRNA
molecules from a single pre-mRNA with a prominent role in the development and function of …

In response to abiotic stress, DNA methylation confers epigenetic changes in plants

Z Akhter, Z Bi, K Ali, C Sun, S Fiaz, FU Haider, J Bai - Plants, 2021 - mdpi.com
Epigenetics involves the heritable changes in patterns of gene expression determined by
developmental and abiotic stresses, ie, drought, cold, salinity, trace metals, and heat. Gene …

Nanopore sequencing of RNA and cDNA molecules in Escherichia coli

F Grünberger, S Ferreira-Cerca, D Grohmann - Rna, 2022 - rnajournal.cshlp.org
High-throughput sequencing dramatically changed our view of transcriptome architectures
and allowed for ground-breaking discoveries in RNA biology. Recently, sequencing of full …

HydraPsiSeq: a method for systematic and quantitative map** of pseudouridines in RNA

V Marchand, F Pichot, P Neybecker… - Nucleic acids …, 2020 - academic.oup.com
Develo** methods for accurate detection of RNA modifications remains a major challenge
in epitranscriptomics. Next-generation sequencing-based map** approaches have …

Advances in mRNA 5-methylcytosine modifications: Detection, effectors, biological functions, and clinical relevance

G Guo, K Pan, S Fang, L Ye, X Tong, Z Wang… - … Therapy-Nucleic Acids, 2021 - cell.com
5-methylcytosine (m 5 C) post-transcriptional modifications affect the maturation, stability,
and translation of the mRNA molecule. These modifications play an important role in many …

New insights into Arabidopsis transcriptome complexity revealed by direct sequencing of native RNAs

S Zhang, R Li, L Zhang, S Chen, M **e… - Nucleic acids …, 2020 - academic.oup.com
Arabidopsis thaliana transcriptomes have been extensively studied and characterized under
different conditions. However, most of the current 'RNA-sequencing'technologies produce a …

Measuring the tail: Methods for poly (A) tail profiling

A Brouze, PS Krawczyk… - Wiley Interdisciplinary …, 2023 - Wiley Online Library
Abstract The 3′‐end poly (A) tail is an important and potent feature of most mRNA
molecules that affects mRNA fate and translation efficiency. Polyadenylation is a …