In-cell structural biology by NMR: the benefits of the atomic scale

FX Theillet - Chemical reviews, 2022 - ACS Publications
In-cell structural biology aims at extracting structural information about proteins or nucleic
acids in their native, cellular environment. This emerging field holds great promise and is …

In-cell NMR: Why and how?

FX Theillet, E Luchinat - Progress in Nuclear Magnetic Resonance …, 2022 - Elsevier
NMR spectroscopy has been applied to cells and tissues analysis since its beginnings, as
early as 1950. We have attempted to gather here in a didactic fashion the broad diversity of …

SLIM: A short‐linked, highly redox‐stable trityl label for high‐sensitivity in‐cell EPR distance measurements

N Fleck, CA Heubach, T Hett, FR Haege… - Angewandte …, 2020 - Wiley Online Library
The understanding of biomolecular function is coupled to knowledge about the structure and
dynamics of these biomolecules, preferably acquired under native conditions. In this regard …

Molecular one‐and two‐qubit systems with very long coherence times

D Schäfter, J Wischnat, L Tesi, JA De Sousa… - Advanced …, 2023 - Wiley Online Library
General‐purpose quantum computation and quantum simulation require multi‐qubit
architectures with precisely defined, robust interqubit interactions, coupled with local …

Impact of cellular crowding on protein structural dynamics investigated by EPR spectroscopy

A Pierro, A Bonucci, A Magalon, V Belle… - Chemical …, 2024 - ACS Publications
The study of how the intracellular medium influences protein structural dynamics and protein–
protein interactions is a captivating area of research for scientists aiming to comprehend …

In‐cell EPR: progress towards structural studies inside cells

A Bonucci, O Ouari, B Guigliarelli, V Belle… - …, 2020 - Wiley Online Library
Exploring the structure and dynamics of biomolecules in the context of their intracellular
environment has become the ultimate challenge for structural biology. As the cellular …

Nanomolar Pulse Dipolar EPR Spectroscopy in Proteins: CuII–CuII and Nitroxide–Nitroxide Cases

K Ackermann, JL Wort, BE Bode - The Journal of Physical …, 2021 - ACS Publications
The study of ever more complex biomolecular assemblies implicated in human health and
disease is facilitated by a suite of complementary biophysical methods. Pulse dipolar …

Pulse dipolar EPR for determining nanomolar binding affinities

K Ackermann, JL Wort, BE Bode - Chemical Communications, 2022 - pubs.rsc.org
Protein interaction studies often require very low concentrations and highly sensitive
biophysical methods. Here, we demonstrate that pulse dipolar electron paramagnetic …

[HTML][HTML] UWB DEER and RIDME distance measurements in Cu (II)–Cu (II) spin pairs

FD Breitgoff, K Keller, M Qi, D Klose, M Yulikov… - Journal of Magnetic …, 2019 - Elsevier
Abstract Distance determination by Electron Paramagnetic Resonance (EPR) based on
measurements of the dipolar coupling are technically challenging for electron spin systems …

Hexanuclear Ln6L6 Complex Formation by Using an Unsymmetric Ligand

DJ Bell, T Zhang, N Geue, CJ Rogers… - … A European Journal, 2023 - Wiley Online Library
Multinuclear, self‐assembled lanthanide complexes present clear opportunities as sensors
and imaging agents. Despite the widely acknowledged potential of this class of …