The role of bioinformatics in two‐dimensional gel electrophoresis

AW Dowsey, MJ Dunn, GZ Yang - PROTEOMICS: International …, 2003 - Wiley Online Library
Over the last two decades, two‐dimensional electrophoresis (2‐DE) gel has established
itself as the de facto approach to separating proteins from cell and tissue samples. Due to …

2D or not 2D

SJ Fey, PM Larsen - Current Opinion in Chemical Biology, 2001 - Elsevier
2D gel electrophoresis is the technology that everyone loves to hate—it requires manual
dexterity and precision to reproduce precisely and is thus not well-suited as a high …

Proteome informatics I: bioinformatics tools for processing experimental data

PM Palagi, P Hernandez, D Walther, RD Appel - Proteomics, 2006 - Wiley Online Library
Bioinformatics tools for proteomics, also called proteome informatics tools, span today a
large panel of very diverse applications ranging from simple tools to compare protein amino …

Hierarchical, model‐based merging of multiple fragments for improved three‐dimensional segmentation of nuclei

G Lin, MK Chawla, K Olson… - Cytometry Part A …, 2005 - Wiley Online Library
Background Automated segmentation of fluorescently labeled cell nuclei in three‐
dimensional confocal images is essential for numerous studies, eg, spatiotemporal …

Proteomic analysis of stromal cells derived from the dental pulp of human exfoliated deciduous teeth

A Pivoriūnas, A Surovas, V Borutinskaitė… - Stem cells and …, 2010 - liebertpub.com
Human dental pulp derived from exfoliated deciduous teeth has been described as a
promising alternative source of multipotent stem cells. While these cells share certain …

[BOK][B] Proteomics today: protein assessment and biomarkers using mass spectrometry, 2D electrophoresis, and microarray technology

MH Hamdan, PG Righetti - 2005 - books.google.com
The last few years have seen an unprecedented drive toward the application of proteomics
to resolving challenging biomedical and biochemical tasks. Separation techniques …

Multiresolution image registration for two‐dimensional gel electrophoresis

S Veeser, MJ Dunn, GZ Yang - PROTEOMICS: International …, 2001 - Wiley Online Library
In proteomic research, two‐dimensional electrophoresis (2‐D) is an important tool for
investigating differential patterns of qualitative and quantitative protein expression. The …

Numerical approaches for quantitative analysis of two‐dimensional maps: A review of commercial software and home‐made systems

E Marengo, E Robotti, F Antonucci, D Cecconi… - …, 2005 - Wiley Online Library
The present review attempts to cover a number of methods that have appeared in the last
few years for performing quantitative proteome analysis. However, due to the large number …

Comparison of PDQuest and Progenesis software packages in the analysis of two‐dimensional electrophoresis gels

AT Rosengren, JM Salmi, T Aittokallio… - …, 2003 - Wiley Online Library
Efficient analysis of protein expression by using two‐dimensional electrophoresis (2‐DE)
data relies on the use of automated image processing techniques. The overall success of …

Analysing proteomic data

J Barrett, PM Brophy, JV Hamilton - International Journal for Parasitology, 2005 - Elsevier
The rapid growth of proteomics has been made possible by the development of reproducible
2D gels and biological mass spectrometry. However, despite technical improvements 2D …