Early work identified two promoter regions, the− 10 and− 35 elements, that interact
sequence specifically with bacterial RNA polymerase (RNAP). However, we now know that …

SUMMARY A major part of organismal complexity and versatility of prokaryotes resides in
their ability to fine-tune gene expression to adequately respond to internal and external …

In Escherichia coli crosstalk between DNA supercoiling, nucleoid-associated proteins and
major RNA polymerase σ initiation factors regulates growth phase-dependent gene …

The formation of viable but nonculturable (VBNC) Escherichia coli O157: H7 induced by
high-pressure CO2 (HPCD) was investigated using RNA sequencing (RNA-Seq) …

In bacteria, RNA polymerase (RNAP) initiates transcription by synthesizing short transcripts
that are either released or extended to allow RNAP to escape from the promoter. The …

Background Transcription elongation is frequently interrupted by pausing signals in DNA,
with downstream effects on gene expression. Transcription errors also induce prolonged …

Cancerous and aging cells have long been thought to be impacted by transcription errors
that cause genetic and epigenetic changes. Until now, a lack of methodology for directly …

Transcription is the first step in gene expression, and its regulation underlies multicellular
development and the response to environmental changes. Most studies of transcriptional …

Initiation is a highly regulated, rate-limiting step in transcription. We used a series of
approaches to examine the kinetics of RNA polymerase (RNAP) transcription initiation in …

The concerted action of ppGpp and DksA in transcription has been widely documented. In
disparity with this model, phenotypic studies showed that ppGpp and DksA might also have …