Recent advances in synthesis of difluoromethylene phosphonates for biological applications
M Shevchuk, Q Wang, R Pajkert, J Xu… - Advanced Synthesis …, 2021 - Wiley Online Library
For almost 40 years, difluoromethylene phosphonates have proven to be versatile molecular
tools in biochemical studies owing to their close resemblance to naturally occurring …
tools in biochemical studies owing to their close resemblance to naturally occurring …
Structure and mechanism of DNA polymerase β
WA Beard, SH Wilson - Biochemistry, 2014 - ACS Publications
DNA polymerase (pol) β is a small eukaryotic DNA polymerase composed of two domains.
Each domain contributes an enzymatic activity (DNA synthesis and deoxyribose phosphate …
Each domain contributes an enzymatic activity (DNA synthesis and deoxyribose phosphate …
How DNA polymerases catalyse replication and repair with contrasting fidelity
DNA polymerases were named for their function of catalysing DNA replication, a process
that is necessary for growth and propagation of life. DNA involving Watson–Crick base …
that is necessary for growth and propagation of life. DNA involving Watson–Crick base …
Trivalent rare earth metal cofactors confer rapid NP-DNA polymerase activity
A DNA polymerase with a single mutation and a divalent calcium cofactor catalyzes the
synthesis of unnatural N3′→ P5′ phosphoramidate (NP) bonds to form NP-DNA …
synthesis of unnatural N3′→ P5′ phosphoramidate (NP) bonds to form NP-DNA …
Nucleotide analog ARL67156 as a lead structure for the development of CD39 and dual CD39/CD73 ectonucleotidase inhibitors
L Schäkel, CC Schmies, RM Idris, X Luo… - Frontiers in …, 2020 - frontiersin.org
Nucleoside triphosphate diphosphohydrolase1 (NTPDase1, CD39) inhibitors have potential
as novel drugs for the (immuno) therapy of cancer. They increase the extracellular …
as novel drugs for the (immuno) therapy of cancer. They increase the extracellular …
A tool for the import of natural and unnatural nucleoside triphosphates into bacteria
AW Feldman, EC Fischer, MP Ledbetter… - Journal of the …, 2018 - ACS Publications
Nucleoside triphosphates play a central role in biology, but efforts to study these roles have
proven difficult because the levels of triphosphates are tightly regulated in a cell and …
proven difficult because the levels of triphosphates are tightly regulated in a cell and …
The catalytic mechanism of DNA and RNA polymerases
DNA and RNA polymerases (Pols) catalyze nucleic acid biosynthesis in all domains of life,
with implications for human diseases and health. Pols carry out nucleic acid extension …
with implications for human diseases and health. Pols carry out nucleic acid extension …
Chemical stabilization of unnatural nucleotide triphosphates for the in vivo expansion of the genetic alphabet
We have developed an unnatural base pair (UBP) and a semisynthetic organism (SSO) that
imports the constituent unnatural nucleoside triphosphates and uses them to replicate DNA …
imports the constituent unnatural nucleoside triphosphates and uses them to replicate DNA …
Modulating the DNA polymerase β reaction equilibrium to dissect the reverse reaction
DD Shock, BD Freudenthal, WA Beard… - Nature chemical …, 2017 - nature.com
DNA polymerases catalyze efficient and high-fidelity DNA synthesis. While this reaction
favors nucleotide incorporation, polymerases also catalyze a reverse reaction …
favors nucleotide incorporation, polymerases also catalyze a reverse reaction …
Modifying the Basicity of the dNTP Leaving Group Modulates Precatalytic Conformational Changes of DNA Polymerase β
The catalytic function of DNA polymerase β (pol β) fulfills the gap-filling requirement of the
base excision DNA repair pathway by incorporating a single nucleotide into a gapped DNA …
base excision DNA repair pathway by incorporating a single nucleotide into a gapped DNA …