Automated in vivo enzyme engineering accelerates biocatalyst optimization

E Orsi, L Schada von Borzyskowski, S Noack… - Nature …, 2024 - nature.com
Achieving cost-competitive bio-based processes requires development of stable and
selective biocatalysts. Their realization through in vitro enzyme characterization and …

A comprehensive review of polyphenol oxidase in tea (Camellia sinensis): Physiological characteristics, oxidation manufacturing, and biosynthesis of functional …

M Tang, S Zhang, L **ong, J Zhou… - … Reviews in Food …, 2023 - Wiley Online Library
Polyphenol oxidase (PPO) is a metalloenzyme with a type III copper core that is abundant in
nature. As one of the most essential enzymes in the tea plant (Camellia sinensis), the further …

YaliCMulti and YaliHMulti: Stable, efficient multi-copy integration tools for engineering Yarrowia lipolytica

M Liu, J Wu, M Yue, Y Ning, X Guan, S Gao… - Metabolic Engineering, 2024 - Elsevier
Yarrowia lipolytica is widely used in biotechnology to produce recombinant proteins, food
ingredients and diverse natural products. However, unstable expression of plasmids, difficult …

Screening non-conventional yeasts for acid tolerance and engineering Pichia occidentalis for production of muconic acid

ME Pyne, JA Bagley, L Narcross, K Kevvai… - Nature …, 2023 - nature.com
Saccharomyces cerevisiae is a workhorse of industrial biotechnology owing to the
organism's prominence in alcohol fermentation and the suite of sophisticated genetic tools …

AutoBioTech─ a versatile biofoundry for automated strain engineering

TM Rosch, J Tenhaef, T Stoltmann… - ACS Synthetic …, 2024 - ACS Publications
The inevitable transition from petrochemical production processes to renewable alternatives
has sparked the emergence of biofoundries in recent years. Manual engineering of …

A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces cerevisiae

WM Shaw, AS Khalil, T Ellis - ACS Synthetic Biology, 2023 - ACS Publications
Synthetic biology toolkits are one of the core foundations on which the field has been built,
facilitating and accelerating efforts to reprogram cells and organisms for diverse …

A roadmap to establish a comprehensive platform for sustainable manufacturing of natural products in yeast

G Naseri - Nature Communications, 2023 - nature.com
Secondary natural products (NPs) are a rich source for drug discovery. However, the low
abundance of NPs makes their extraction from nature inefficient, while chemical synthesis is …

Modularized synthetic biology enabled intelligent biosensors

C Zhang, H Liu, X Li, F Xu, Z Li - Trends in Biotechnology, 2023 - cell.com
Biosensors that sense the concentration of a specified target and produce a specific signal
output have become important technology for biological analysis. Recently, intelligent …

High‐complexity one‐pot Golden Gate assembly

AP Sikkema, SK Tabatabaei, YJ Lee, S Lund… - Current …, 2023 - Wiley Online Library
Abstract Golden Gate Assembly is a flexible method of DNA assembly and cloning that
permits the joining of multiple fragments in a single reaction through predefined …

Improved production of Taxol® precursors in S. cerevisiae using combinatorial in silico design and metabolic engineering

K Malcı, R Santibáñez, N Jonguitud-Borrego… - Microbial Cell …, 2023 - Springer
Background Integrated metabolic engineering approaches that combine system and
synthetic biology tools enable the efficient design of microbial cell factories for synthesizing …