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Automated in vivo enzyme engineering accelerates biocatalyst optimization
Achieving cost-competitive bio-based processes requires development of stable and
selective biocatalysts. Their realization through in vitro enzyme characterization and …
selective biocatalysts. Their realization through in vitro enzyme characterization and …
A comprehensive review of polyphenol oxidase in tea (Camellia sinensis): Physiological characteristics, oxidation manufacturing, and biosynthesis of functional …
M Tang, S Zhang, L **ong, J Zhou… - … Reviews in Food …, 2023 - Wiley Online Library
Polyphenol oxidase (PPO) is a metalloenzyme with a type III copper core that is abundant in
nature. As one of the most essential enzymes in the tea plant (Camellia sinensis), the further …
nature. As one of the most essential enzymes in the tea plant (Camellia sinensis), the further …
YaliCMulti and YaliHMulti: Stable, efficient multi-copy integration tools for engineering Yarrowia lipolytica
M Liu, J Wu, M Yue, Y Ning, X Guan, S Gao… - Metabolic Engineering, 2024 - Elsevier
Yarrowia lipolytica is widely used in biotechnology to produce recombinant proteins, food
ingredients and diverse natural products. However, unstable expression of plasmids, difficult …
ingredients and diverse natural products. However, unstable expression of plasmids, difficult …
Screening non-conventional yeasts for acid tolerance and engineering Pichia occidentalis for production of muconic acid
Saccharomyces cerevisiae is a workhorse of industrial biotechnology owing to the
organism's prominence in alcohol fermentation and the suite of sophisticated genetic tools …
organism's prominence in alcohol fermentation and the suite of sophisticated genetic tools …
AutoBioTech─ a versatile biofoundry for automated strain engineering
TM Rosch, J Tenhaef, T Stoltmann… - ACS Synthetic …, 2024 - ACS Publications
The inevitable transition from petrochemical production processes to renewable alternatives
has sparked the emergence of biofoundries in recent years. Manual engineering of …
has sparked the emergence of biofoundries in recent years. Manual engineering of …
A Multiplex MoClo Toolkit for Extensive and Flexible Engineering of Saccharomyces cerevisiae
Synthetic biology toolkits are one of the core foundations on which the field has been built,
facilitating and accelerating efforts to reprogram cells and organisms for diverse …
facilitating and accelerating efforts to reprogram cells and organisms for diverse …
A roadmap to establish a comprehensive platform for sustainable manufacturing of natural products in yeast
G Naseri - Nature Communications, 2023 - nature.com
Secondary natural products (NPs) are a rich source for drug discovery. However, the low
abundance of NPs makes their extraction from nature inefficient, while chemical synthesis is …
abundance of NPs makes their extraction from nature inefficient, while chemical synthesis is …
Modularized synthetic biology enabled intelligent biosensors
Biosensors that sense the concentration of a specified target and produce a specific signal
output have become important technology for biological analysis. Recently, intelligent …
output have become important technology for biological analysis. Recently, intelligent …
High‐complexity one‐pot Golden Gate assembly
Abstract Golden Gate Assembly is a flexible method of DNA assembly and cloning that
permits the joining of multiple fragments in a single reaction through predefined …
permits the joining of multiple fragments in a single reaction through predefined …
Improved production of Taxol® precursors in S. cerevisiae using combinatorial in silico design and metabolic engineering
K Malcı, R Santibáñez, N Jonguitud-Borrego… - Microbial Cell …, 2023 - Springer
Background Integrated metabolic engineering approaches that combine system and
synthetic biology tools enable the efficient design of microbial cell factories for synthesizing …
synthetic biology tools enable the efficient design of microbial cell factories for synthesizing …