Cryo-electron tomography: the resolution revolution and a surge of in situ virological discoveries

Y Hong, Y Song, Z Zhang, S Li - Annual Review of Biophysics, 2023 - annualreviews.org
The recent proliferation of cryo-electron tomography (cryo-ET) techniques has led to the cryo-
ET resolution revolution. Meanwhile, significant efforts have been made to improve the …

Molecular architecture of coronavirus double-membrane vesicle pore complex

Y Huang, T Wang, L Zhong, W Zhang, Y Zhang, X Yu… - Nature, 2024 - nature.com
Coronaviruses remodel the intracellular host membranes during replication, forming double-
membrane vesicles (DMVs) to accommodate viral RNA synthesis and modifications,. SARS …

Cryo-electron tomography to study viral infection

M Graham, P Zhang - Biochemical Society Transactions, 2023 - portlandpress.com
Developments in cryo-electron microscopy (cryo-EM) have been interwoven with the study
of viruses ever since its first applications to biological systems. Following the success of …

Alphavirus nsP3 organizes into tubular scaffolds essential for infection and the cytoplasmic granule architecture

V Kril, M Hons, C Amadori, C Zimberger… - Nature …, 2024 - nature.com
Alphaviruses, such as chikungunya virus (CHIKV), are mosquito-borne viruses that
represent a significant threat to human health due to the current context of global warming …

[HTML][HTML] Alphavirus infection triggers selective cytoplasmic translocation of nuclear RBPs with moonlighting antiviral roles

W Kamel, V Ruscica, A Embarc-Buh, ZR de Laurent… - Molecular Cell, 2024 - cell.com
RNA is a central molecule for viruses; however, the interactions that viral RNA (vRNA)
establishes with the host cell is only starting to be elucidated. Here, we determine the …

Structural basis and dynamics of Chikungunya alphavirus RNA cap** by nsP1 cap** pores

R Jones, M Hons, N Rabah… - Proceedings of the …, 2023 - National Acad Sciences
Alphaviruses are emerging positive-stranded RNA viruses which replicate and transcribe
their genomes in membranous organelles formed in the cell cytoplasm. The nonstructural …

Nodavirus RNA replication crown architecture reveals proto-crown precursor and viral protein A conformational switching

H Zhan, N Unchwaniwala… - Proceedings of the …, 2023 - National Acad Sciences
Positive-strand RNA viruses replicate their genomes in virus-induced membrane vesicles,
and the resulting RNA replication complexes are a major target for virus control. Nodavirus …

In situ fate of Chikungunya virus replication organelles

J Girard, O Le Bihan, J Lai-Kee-Him… - Journal of …, 2024 - journals.asm.org
Chikungunya virus (CHIKV) is a mosquito-borne pathogen responsible for an acute
musculoskeletal disease in humans. Replication of the viral RNA genome occurs in …

The evolutionary and molecular history of a chikungunya virus outbreak lineage

J Krambrich, F Mihalič, MW Gaunt… - PLOS Neglected …, 2024 - journals.plos.org
In 2018–2019, Thailand experienced a nationwide spread of chikungunya virus (CHIKV),
with approximately 15,000 confirmed cases of disease reported. Here, we investigated the …

Exploring Barmah Forest virus pathogenesis: molecular tools to investigate non-structural protein 3 nuclear localization and viral genomic determinants of replication

A Omler, M Mutso, M Vaher, JR Freitas, A Taylor… - Mbio, 2024 - journals.asm.org
Barmah Forest virus (BFV) is a mosquito-borne virus that causes arthralgia with
accompanying rash, fever, and myalgia in humans. The virus is mainly found in Australia …