Trehalose in cryopreservation. Applications, mechanisms and intracellular delivery opportunities

A Murray, P Kilbride, MI Gibson - RSC Medicinal Chemistry, 2024 - pubs.rsc.org
Cryopreservation is crucial to fields including immune and stem cell therapies, reproductive
technology, blood banking, regenerative medicine and across all biotechnology. During …

Late embryogenesis abundant proteins protect human hepatoma cells during acute desiccation

S Li, N Chakraborty, A Borcar… - Proceedings of the …, 2012 - National Acad Sciences
Expression of late embryogenesis abundant (LEA) proteins is highly correlated with
desiccation tolerance in anhydrobiotic animals, selected land plants, and bacteria. Genes …

Technologies and applications toward preservation of cells in a dry state for therapies

L Weng - Biopreservation and Biobanking, 2021 - liebertpub.com
Cell-based therapeutics promise to transform the treatment of a wide range of diseases,
many of which, up to this point, are incurable. During the past decade, an increasing number …

Cryopreservation of hepatocyte (HepG2) cell monolayers: impact of trehalose

B Stokich, Q Osgood, D Grimm, S Moorthy… - Cryobiology, 2014 - Elsevier
A simple method to cryogenically preserve hepatocyte monolayers is currently not available
but such a technique would facilitate numerous applications in the field of biomedical …

Protective effects of osmolytes in cryopreserving adherent neuroblastoma (Neuro-2a) cells

TL Bailey, M Wang, J Solocinski, BP Nathan… - Cryobiology, 2015 - Elsevier
A simple method to cryopreserve adherent monolayers of neuronal cells is currently not
available, but the development of this technique could facilitate numerous applications in the …

Preserving the female genome in trehalose glass at supra-zero temperatures: The relationship between moisture content and DNA damage in feline germinal vesicles

S Wang, PC Lee, A Elsayed, F Zhang, Y Zhang… - Cellular and Molecular …, 2021 - Springer
Introduction Maintaining a stable dry state is critical for long-term preservation of live
biomaterials at suprazero temperatures. The objective of the study was to characterize the …

Determination of water content in dehydrated mammalian cells using terahertz pulsed imaging: a feasibility study

D YS Chau, AR Dennis, H Lin… - Current …, 2016 - ingentaconnect.com
Mammalian cells are involved in a range of biotechnological applications and more recently
have been increasingly exploited in regenerative medicine. Critical to successful …

A Raman microspectroscopy study of water and trehalose in spin-dried cells

A Abazari, N Chakraborty, S Hand, A Aksan, M Toner - Biophysical journal, 2014 - cell.com
Long-term storage of desiccated nucleated mammalian cells at ambient temperature may be
accomplished in a stable glassy state, which can be achieved by removal of water from the …

Dry storage of mammalian spermatozoa and cells: state-of-the-art and possible future directions

P Loi, DA Anzalone, L Palazzese… - Reproduction …, 2021 - CSIRO Publishing
This review provides a snapshot of the current state-of-the-art of drying cells and
spermatozoa. The major successes and pitfalls of the most relevant literature are described …

DMSO as new, counterintuitive excipient for freeze-drying human keratinocytes

U Rockinger, C Müller, F Bracher, M Funk… - European Journal of …, 2021 - Elsevier
DMSO is widely used as powerful cryoprotectant for the storage and transport of frozen cells.
Beyond this established application of DMSO, we could now show that it has also promising …