Replication fork reversal and protection

S Qiu, G Jiang, L Cao, J Huang - Frontiers in cell and developmental …, 2021 - frontiersin.org
During genome replication, replication forks often encounter obstacles that impede their
progression. Arrested forks are unstable structures that can give rise to collapse and …

Making choices: DNA replication fork recovery mechanisms

CM Kondratick, MT Washington, M Spies - Seminars in cell & …, 2021 - Elsevier
DNA replication is laden with obstacles that slow, stall, collapse, and break DNA replication
forks. At each obstacle, there is a decision to be made whether to bypass the lesion, repair …

[HTML][HTML] CTCF and R-loops are boundaries of cohesin-mediated DNA loo**

H Zhang, Z Shi, EJ Banigan, Y Kim, H Yu, X Bai… - Molecular cell, 2023 - cell.com
Cohesin and CCCTC-binding factor (CTCF) are key regulatory proteins of three-dimensional
(3D) genome organization. Cohesin extrudes DNA loops that are anchored by CTCF in a …

Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9

GN Hibshman, JPK Bravo, MM Hooper… - Nature …, 2024 - nature.com
CRISPR-Cas9 is a powerful tool for genome editing, but the strict requirement for an NGG
protospacer-adjacent motif (PAM) sequence immediately next to the DNA target limits the …

FIRRM/C1orf112 is synthetic lethal with PICH and mediates RAD51 dynamics

C Stok, S Tsaridou, N van den Tempel, M Everts… - Cell reports, 2023 - cell.com
Joint DNA molecules are natural byproducts of DNA replication and repair. Persistent joint
molecules give rise to ultrafine DNA bridges (UFBs) in mitosis, compromising sister …

How glutamate promotes liquid-liquid phase separation and DNA binding cooperativity of E. coli SSB protein

AG Kozlov, X Cheng, H Zhang, MK Shinn… - Journal of molecular …, 2022 - Elsevier
E. coli single-stranded-DNA binding protein (EcSSB) displays nearest-neighbor (NN) and
non-nearest-neighbor (NNN)) cooperativity in binding ssDNA during genome maintenance …

Polymerase theta-helicase promotes end joining by strip** single-stranded DNA-binding proteins and bridging DNA ends

JM Schaub, MM Soniat, IJ Finkelstein - Nucleic acids research, 2022 - academic.oup.com
Homologous recombination-deficient cancers rely on DNA polymerase Theta (Polθ)-
Mediated End Joining (TMEJ), an alternative double-strand break repair pathway. Polθ is …

To join or not to join: decision points along the pathway to double-strand break repair vs. chromosome end protection

SM Ackerson, C Romney, PL Schuck… - Frontiers in cell and …, 2021 - frontiersin.org
The regulation of DNA double-strand breaks (DSBs) and telomeres are diametrically
opposed in the cell. DSBs are considered one of the most deleterious forms of DNA damage …

[HTML][HTML] RTEL1 helicase counteracts RAD51-mediated homologous recombination and fork reversal to safeguard replicating genomes

S Dixit, T Nagraj, D Bhattacharya, S Saxena, S Sahoo… - Cell Reports, 2024 - cell.com
Homologous recombination (HR) plays an essential role in the repair of DNA double-strand
breaks (DSBs), replication stress responses, and genome maintenance. However …

RADX controls RAD51 filament dynamics to regulate replication fork stability

MB Adolph, TM Mohamed, S Balakrishnan, C Xue… - Molecular cell, 2021 - cell.com
The RAD51 recombinase forms nucleoprotein filaments to promote double-strand break
repair, replication fork reversal, and fork stabilization. The stability of these filaments is highly …