For decades, the spatial resolution of conventional far‐field optical imaging has been
constrained due to the diffraction limit. The emergence of optical super‐resolution imaging …

Stimulated emission depletion (STED) microscopy is a useful tool in investigation for super‐
resolution realm. By silencing the peripheral fluorophores of the excited spot, leaving only …

We demonstrate that the spatial resolution in confocal laser scanning microscopy is
remarkably improved by simple image subtraction between images acquired by higher …

Time-gated detection, namely, only collecting the fluorescence photons after a time-delay
from the excitation events, reduces complexity, cost, and illumination intensity of a stimulated …

We have designed and built a time-gated continuous wave stimulated emission depletion
(CW-STED) nanoscopy to visualize microstructures beyond the diffraction limit. An off-line …

Point spread function (PSF) engineering-based methods to enhance resolution and contrast
of optical microscopes have experienced great achievements in the last decades. These …

The recently proposed fluorescence emission difference (FED) microscopy has been
demonstrated to be capable of breaking the diffraction barrier, which restricts the spatial …

A new method based on the subtraction of images is proposed to enhance the resolution
and contrast in CW-STED microscopy. It is realized by the subtraction of a confocal image …

Method of optical microscopy by scanning a sample con taining an excitable species, the
method comprising: directing a first and a second light beam onto respective, partially …

The resolution of conventional optical microscopy is constrained to about 200 and 500 nm in
the lateral and axial plane, respectively, because diffraction sets a physical limit for the …