Can invalid bioactives undermine natural product-based drug discovery?
High-throughput biology has contributed a wealth of data on chemicals, including natural
products (NPs). Recently, attention was drawn to certain, predominantly synthetic …
products (NPs). Recently, attention was drawn to certain, predominantly synthetic …
The essential roles of chemistry in high-throughput screening triage
It is increasingly clear that academic high-throughput screening (HTS) and virtual HTS triage
suffers from a lack of scientists trained in the art and science of early drug discovery …
suffers from a lack of scientists trained in the art and science of early drug discovery …
A rapid cell-free expression and screening platform for antibody discovery
Antibody discovery is bottlenecked by the individual expression and evaluation of antigen-
specific hits. Here, we address this bottleneck by develo** a workflow combining cell-free …
specific hits. Here, we address this bottleneck by develo** a workflow combining cell-free …
PAINS in the assay: chemical mechanisms of assay interference and promiscuous enzymatic inhibition observed during a sulfhydryl-scavenging HTS
Significant resources in early drug discovery are spent unknowingly pursuing artifacts and
promiscuous bioactive compounds, while understanding the chemical basis for these …
promiscuous bioactive compounds, while understanding the chemical basis for these …
Phantom PAINS: Problems with the Utility of Alerts for P an-A ssay IN terference Compound S
The use of substructural alerts to identify P an-A ssay IN terference compound S (PAINS)
has become a common component of the triage process in biological screening campaigns …
has become a common component of the triage process in biological screening campaigns …
BIGCHEM: challenges and opportunities for big data analysis in chemistry
The increasing volume of biomedical data in chemistry and life sciences requires the
development of new methods and approaches for their handling. Here, we briefly discuss …
development of new methods and approaches for their handling. Here, we briefly discuss …
Current experimental methods for characterizing protein–protein interactions
M Zhou, Q Li, R Wang - ChemMedChem, 2016 - Wiley Online Library
Protein molecules often interact with other partner protein molecules in order to execute their
vital functions in living organisms. Characterization of protein–protein interactions thus plays …
vital functions in living organisms. Characterization of protein–protein interactions thus plays …
Evolution of Novartis' small molecule screening deck design
This article summarizes the evolution of the screening deck at the Novartis Institutes for
BioMedical Research (NIBR). Historically, the screening deck was an assembly of all …
BioMedical Research (NIBR). Historically, the screening deck was an assembly of all …
Using microscale thermophoresis to characterize hits from high-throughput screening: a European lead factory perspective
JM Rainard, GC Pandarakalam… - … Life Sciences R&D, 2018 - journals.sagepub.com
High-throughput screening (HTS) is a proven method for discovering new lead matter for
drug discovery and chemical biology. To maximize the likelihood of identifying genuine …
drug discovery and chemical biology. To maximize the likelihood of identifying genuine …
Nuisance compounds in cellular assays
JL Dahlin, DS Auld, I Rothenaigner, S Haney… - Cell chemical …, 2021 - cell.com
Compounds that exhibit assay interference or undesirable mechanisms of bioactivity ("
nuisance compounds") are routinely encountered in cellular assays, including phenotypic …
nuisance compounds") are routinely encountered in cellular assays, including phenotypic …