As an indispensable component of recombinant fusion proteins, linkers have shown
increasing importance in the construction of stable, bioactive fusion proteins. This review …

Proline-rich regions (PRRs) of proteins occur widely in both prokaryotes and eukaryotes.
They are frequently found as multiple tandem repeats, often of considerable length. Despite …

The greatly improved prediction of protein 3D structure from sequence achieved by the
second version of AlphaFold in 2020 has already had a huge impact on biological research …

Abstract Knowledge of protein structure can be used to predict the phenotypic consequence
of a missense variant. Since structural coverage of the human proteome can be roughly …

Comparative protein modeling is increasingly gaining interest since it is of great assistance
during the rational design of mutagenesis experiments. The availability of this method, and …

The PROCHECK suite of programs provides a detailed check on the stereochemistry of a
protein structure. Its outputs comprise a number of plots in PostScript format and a …

Geometrical validation around the C is described, with a new C measure and updated
Ramachandran plot. Deviation of the observed C atom from ideal position provides a single …

Summary The AQUA and PROCHECK-NMR programs provide a means of validating the
geometry and restraint violations of an ensemble of protein structures solved by solution …

We have developed an automatic algorithm STRIDE for protein secondary structure
assignment from atomic coordinates based on the combined use of hydrogen bond energy …

We have analysed the frequency with which potential hydrogen bond donors and acceptors
are satisfied in protein molecules. There are a small percentage of nitrogen or oxygen atoms …