Applications of environmental DNA (eDNA) in ecology and conservation: opportunities, challenges and prospects

KC Beng, RT Corlett - Biodiversity and conservation, 2020 - Springer
Conserving biodiversity in the face of ever-increasing human pressure is hampered by our
lack of basic information on species occurrence, distribution, abundance, habitat …

Environmental DNA metabarcoding: A novel method for biodiversity monitoring of marine fish communities

M Miya - Annual Review of Marine Science, 2022 - annualreviews.org
Environmental DNA (eDNA) is genetic material that has been shed from macroorganisms. It
has received increased attention as an indirect marker for biodiversity monitoring. This …

Meta‐analysis shows both congruence and complementarity of DNA and eDNA metabarcoding to traditional methods for biological community assessment

F Keck, RC Blackman, R Bossart… - Molecular …, 2022 - Wiley Online Library
DNA metabarcoding is increasingly used for the assessment of aquatic communities, and
numerous studies have investigated the consistency of this technique with traditional …

MiFish metabarcoding: a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples

M Miya, RO Gotoh, T Sado - Fisheries Science, 2020 - Springer
We reviewed the current methodology and practices of the DNA metabarcoding approach
using a universal PCR primer pair MiFish, which co-amplifies a short fragment of fish DNA …

Lake sedimentary DNA research on past terrestrial and aquatic biodiversity: Overview and recommendations

E Capo, C Giguet-Covex, A Rouillard, K Nota… - Quaternary, 2021 - mdpi.com
The use of lake sedimentary DNA to track the long-term changes in both terrestrial and
aquatic biota is a rapidly advancing field in paleoecological research. Although largely …

Fishing for fish environmental DNA: Ecological applications, methodological considerations, surveying designs, and ways forward

M Yao, S Zhang, Q Lu, X Chen, SY Zhang… - Molecular …, 2022 - Wiley Online Library
Vast global declines of freshwater and marine fish diversity and population abundance pose
serious threats to both ecosystem sustainability and human livelihoods. Environmental DNA …

Methodology of fish eDNA and its applications in ecology and environment

S Wang, Z Yan, B Hänfling, X Zheng, P Wang… - Science of the Total …, 2021 - Elsevier
Fish environmental DNA (eDNA) studies have made substantial progress during the past
decade, and significant advances in monitoring fishes have been gained by taking …

An illustrated manual for environmental DNA research: Water sampling guidelines and experimental protocols

T Minamoto, M Miya, T Sado, S Seino, H Doi… - Environmental …, 2021 - Wiley Online Library
Environmental DNA (eDNA)‐based assessments of macro‐organisms have now become an
essential approach for biomonitoring. eDNA survey methods have a number of advantages …

Validation of COI metabarcoding primers for terrestrial arthropods

V Elbrecht, TWA Braukmann, NV Ivanova, SWJ Prosser… - PeerJ, 2019 - peerj.com
Metabarcoding can rapidly determine the species composition of bulk samples and thus
aids biodiversity and ecosystem assessment. However, it is essential to use primer sets that …

MIDORI2: A collection of quality controlled, preformatted, and regularly updated reference databases for taxonomic assignment of eukaryotic mitochondrial sequences

M Leray, N Knowlton, RJ Machida - Environmental DNA, 2022 - Wiley Online Library
Abstract Analysis of environmental DNA is increasingly used to characterize ecological
communities, but the effectiveness of this approach depends on the accuracy of taxonomic …