The ability to design efficient enzymes from scratch would have a profound effect on
chemistry, biotechnology and medicine. Rapid progress in protein engineering over the past …

In the early 2000s, Tawfik presented his 'New View'on enzyme evolution, highlighting the
role of conformational plasticity in expanding the functional diversity of limited repertoires of …

Regenerable nanozymes with high catalytic stability and sustainability are promising
substitutes for naturally-occurring enzymes but are limited by insufficient and non-selective …

The design and improvement of enzymes based on physical principles remain challenging.
Here we demonstrate that the principle of electrostatic catalysis can be leveraged to …

Ketones are crucial intermediates in synthesis and frequent moieties in many products. The
direct regioselective synthesis of ketones from internal alkenes could simplify synthetic …

Epistasis, the non-additive effect of mutations, can provide combinatorial improvements to
enzyme activity that substantially exceed the gains from individual mutations. Yet the …

In nature, the oxygen reduction reaction (ORR) is catalyzed by cytochrome P450 (CYP)
enzymes containing heme iron centers with an axial thiolate ligand (FeN4‐S), which are …

Resistance to antibiotics is a public health crisis. Although carbapenems are less
susceptible to resistance than other β-lactam antibiotics, β-lactamases mediating resistance …

The widespread trade-off between stability and activity severely limits enzyme evolution.
Although some progresses have been made to overcome this limitation, the counteraction …

Protein functions are dynamically regulated by allostery, which enables conformational
communication even between faraway residues, and expresses itself in many forms, akin to …