Antibody–drug conjugates (ADCs) harness the highly specific targeting capabilities of an
antibody to deliver a cytotoxic payload to specific cell types. They have garnered …

The present article comprehensively reviews the macromolecular synthesis using enzymes
as catalysts. Among the six main classes of enzymes, the three classes, oxidoreductases …

Here, we review the current state of the art in analytical and structural methods used to
characterize therapeutic monoclonal antibodies (mAbs) and derivatives. First, a general …

Background Glycoproteins and glycolipids exist as an ensemble of glycosylated variants, or
glycoforms. Specific glycoforms are directly modulated by microenvironmental cues and play …

Glycosylation is one of the most prevalent posttranslational modifications that profoundly
affects the structure and functions of proteins in a wide variety of biological recognition …

The translation of biological glycosylation in humans to the clinical applications involves
systematic studies using homogeneous samples of oligosaccharides and glycoconjugates …

The fine structures of Fc N-glycans can modulate the effector functions of IgG antibodies. It
has been demonstrated that lack of the core fucose on the Fc N-glycans leads to drastic …

A robust, generally applicable, nongenetic technology is presented to convert monoclonal
antibodies into stable and homogeneous ADCs. Starting from a native (nonengineered) …

Antibodies have been developed as therapeutic agents for the treatment of cancer, infection,
and inflammation. In addition to binding activity toward the target, antibodies also exhibit …

The endo-β-N-acetylglucosaminidases (ENGases) are an enzyme class (EC 3.2. 1.96)
produced by a range of organisms, ranging from bacteria, through fungi, to higher order …