Evolution of mammalian chitinase (-like) members of family 18 glycosyl hydrolases

AP Bussink, D Speijer, JMFG Aerts, RG Boot - Genetics, 2007 - academic.oup.com
Family 18 of glycosyl hydrolases encompasses chitinases and so-called chi-lectins lacking
enzymatic activity due to amino acid substitutions in their active site. Both types of proteins …

Sequence and structural analysis of the chitinase insertion domain reveals two conserved motifs involved in chitin-binding

H Li, LH Greene - Plos one, 2010 - journals.plos.org
Background Chitinases are prevalent in life and are found in species including archaea,
bacteria, fungi, plants, and animals. They break down chitin, which is the second most …

Modification and periplasmic translocation of the biofilm exopolysaccharide poly-β-1,6-N-acetyl-d-glucosamine

DJ Little, G Li, C Ing, BR DiFrancesco… - Proceedings of the …, 2014 - pnas.org
Poly-β-1, 6-N-acetyl-d-glucosamine (PNAG) is an exopolysaccharide produced by a wide
variety of medically important bacteria. Polyglucosamine subunit B (PgaB) is responsible for …

Crystal structures of Vibrio harveyi chitinase A complexed with chitooligosaccharides: implications for the catalytic mechanism

C Songsiriritthigul, S Pantoom, AH Aguda… - Journal of structural …, 2008 - Elsevier
This research describes four X-ray structures of Vibrio harveyi chitinase A and its
catalytically inactive mutant (E315M) in the presence and absence of substrates. The overall …

Purification, sequence characterization and effect of goat oviduct-specific glycoprotein on in vitro embryo development

MA Pradeep, J Jagadeesh, AK De, JK Kaushik… - Theriogenology, 2011 - Elsevier
Oviduct-specific glycoprotein (oviductin) plays an important role during fertilization and early
embryonic development. The oviductin cDNA was successfully cloned and sequenced in …

Structural and functional characterization of buffalo oviduct-specific glycoprotein (OVGP1) expressed during estrous cycle

S Choudhary, J Janjanam, S Kumar… - Bioscience …, 2019 - portlandpress.com
Abstract Oviduct-specific glycoprotein (OVGP1) is a high molecular weight chitinase-like
protein belonging to GH18 family. It is secreted by non-ciliated epithelial cells of oviduct …

Milk yield responses to changes in milking frequency during early lactation are associated with coordinated and persistent changes in mammary gene expression

EH Wall, JP Bond, TB McFadden - BMC genomics, 2013 - Springer
Background The lactating mammary gland responds to changes in milking frequency by
modulating milk production. This response is locally regulated and, in dairy cows, the udder …

Insights into the role of the (α+ β) insertion in the TIM-barrel catalytic domain, regarding the stability and the enzymatic activity of Chitinase A from Serratia marcescens

AC Zees, S Pyrpassopoulos, CE Vorgias - Biochimica et Biophysica Acta …, 2009 - Elsevier
Chitinase A (ChiA) from Serratia marcescens is a mesophilic enzyme with high catalytic
activity and high stability. The crystal structure of ChiA has revealed a TIM-barrel fold of the …

Cloning, expression and purification of the outer membrane protein N from Gram-negative bacterial strains

W Chumjan, P Wiboongun, K Muangcham… - Process …, 2023 - Elsevier
To employ polyclonal antibodies against 6his-tags to detect the expression of recombinant
EcOmpN in the E. coli BL21 (DE3) Omp8 Rosetta strain using a Western blot, the ompN …

Chitoporin from the marine bacterium Vibrio harveyi: probing the essential roles of Trp136 at the surface of the constriction zone

W Chumjan, M Winterhalter, A Schulte, R Benz… - Journal of Biological …, 2015 - jbc.org
VhChiP is a sugar-specific porin present in the outer membrane of the marine bacterium
Vibrio harveyi. VhChiP is responsible for the uptake of chitin oligosaccharides, with …