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Evolution of mammalian chitinase (-like) members of family 18 glycosyl hydrolases
Family 18 of glycosyl hydrolases encompasses chitinases and so-called chi-lectins lacking
enzymatic activity due to amino acid substitutions in their active site. Both types of proteins …
enzymatic activity due to amino acid substitutions in their active site. Both types of proteins …
Sequence and structural analysis of the chitinase insertion domain reveals two conserved motifs involved in chitin-binding
H Li, LH Greene - Plos one, 2010 - journals.plos.org
Background Chitinases are prevalent in life and are found in species including archaea,
bacteria, fungi, plants, and animals. They break down chitin, which is the second most …
bacteria, fungi, plants, and animals. They break down chitin, which is the second most …
Modification and periplasmic translocation of the biofilm exopolysaccharide poly-β-1,6-N-acetyl-d-glucosamine
Poly-β-1, 6-N-acetyl-d-glucosamine (PNAG) is an exopolysaccharide produced by a wide
variety of medically important bacteria. Polyglucosamine subunit B (PgaB) is responsible for …
variety of medically important bacteria. Polyglucosamine subunit B (PgaB) is responsible for …
Crystal structures of Vibrio harveyi chitinase A complexed with chitooligosaccharides: implications for the catalytic mechanism
C Songsiriritthigul, S Pantoom, AH Aguda… - Journal of structural …, 2008 - Elsevier
This research describes four X-ray structures of Vibrio harveyi chitinase A and its
catalytically inactive mutant (E315M) in the presence and absence of substrates. The overall …
catalytically inactive mutant (E315M) in the presence and absence of substrates. The overall …
Purification, sequence characterization and effect of goat oviduct-specific glycoprotein on in vitro embryo development
Oviduct-specific glycoprotein (oviductin) plays an important role during fertilization and early
embryonic development. The oviductin cDNA was successfully cloned and sequenced in …
embryonic development. The oviductin cDNA was successfully cloned and sequenced in …
Structural and functional characterization of buffalo oviduct-specific glycoprotein (OVGP1) expressed during estrous cycle
Abstract Oviduct-specific glycoprotein (OVGP1) is a high molecular weight chitinase-like
protein belonging to GH18 family. It is secreted by non-ciliated epithelial cells of oviduct …
protein belonging to GH18 family. It is secreted by non-ciliated epithelial cells of oviduct …
Milk yield responses to changes in milking frequency during early lactation are associated with coordinated and persistent changes in mammary gene expression
EH Wall, JP Bond, TB McFadden - BMC genomics, 2013 - Springer
Background The lactating mammary gland responds to changes in milking frequency by
modulating milk production. This response is locally regulated and, in dairy cows, the udder …
modulating milk production. This response is locally regulated and, in dairy cows, the udder …
Insights into the role of the (α+ β) insertion in the TIM-barrel catalytic domain, regarding the stability and the enzymatic activity of Chitinase A from Serratia marcescens
AC Zees, S Pyrpassopoulos, CE Vorgias - Biochimica et Biophysica Acta …, 2009 - Elsevier
Chitinase A (ChiA) from Serratia marcescens is a mesophilic enzyme with high catalytic
activity and high stability. The crystal structure of ChiA has revealed a TIM-barrel fold of the …
activity and high stability. The crystal structure of ChiA has revealed a TIM-barrel fold of the …
Cloning, expression and purification of the outer membrane protein N from Gram-negative bacterial strains
W Chumjan, P Wiboongun, K Muangcham… - Process …, 2023 - Elsevier
To employ polyclonal antibodies against 6his-tags to detect the expression of recombinant
EcOmpN in the E. coli BL21 (DE3) Omp8 Rosetta strain using a Western blot, the ompN …
EcOmpN in the E. coli BL21 (DE3) Omp8 Rosetta strain using a Western blot, the ompN …
Chitoporin from the marine bacterium Vibrio harveyi: probing the essential roles of Trp136 at the surface of the constriction zone
VhChiP is a sugar-specific porin present in the outer membrane of the marine bacterium
Vibrio harveyi. VhChiP is responsible for the uptake of chitin oligosaccharides, with …
Vibrio harveyi. VhChiP is responsible for the uptake of chitin oligosaccharides, with …