What macromolecular crowding can do to a protein
The intracellular environment represents an extremely crowded milieu, with a limited amount
of free water and an almost complete lack of unoccupied space. Obviously, slightly salted …
of free water and an almost complete lack of unoccupied space. Obviously, slightly salted …
Models of macromolecular crowding effects and the need for quantitative comparisons with experiment
AH Elcock - Current opinion in structural biology, 2010 - Elsevier
In recent years significant effort has been devoted to exploring the potential effects of
macromolecular crowding on protein folding and association phenomena. Theoretical …
macromolecular crowding on protein folding and association phenomena. Theoretical …
Hydrogen bonding steers the product selectivity of electrocatalytic CO reduction
The product selectivity of many heterogeneous electrocatalytic processes is profoundly
affected by the liquid side of the electrocatalytic interface. The electrocatalytic reduction of …
affected by the liquid side of the electrocatalytic interface. The electrocatalytic reduction of …
Macromolecular crowding remodels the energy landscape of a protein by favoring a more compact unfolded state
The interior of cells is highly crowded with macromolecules, which impacts all physiological
processes. To explore how macromolecular crowding may influence cellular protein folding …
processes. To explore how macromolecular crowding may influence cellular protein folding …
Size-dependent studies of macromolecular crowding on the thermodynamic stability, structure and functional activity of proteins: in vitro and in silico approaches
Background The environment inside cells in which proteins fold and function are quite
different from that of the dilute buffer solutions often used during in vitro experiments. The …
different from that of the dilute buffer solutions often used during in vitro experiments. The …
Crowders and cosolvents—major contributors to the cellular milieu and efficient means to counteract environmental stresses
The free energy and conformational landscape of biomolecular systems as well as
biochemical reactions depend not only on temperature and pressure, but also on the …
biochemical reactions depend not only on temperature and pressure, but also on the …
Temperature dependence of protein folding kinetics in living cells
We measure the stability and folding rate of a mutant of the enzyme phosphoglycerate
kinase (PGK) inside bone tissue cells as a function of temperature from 38 to 48° C. To …
kinase (PGK) inside bone tissue cells as a function of temperature from 38 to 48° C. To …
Protein stability and folding kinetics in the nucleus and endoplasmic reticulum of eucaryotic cells
We measure the stability and folding relaxation rate of phosphoglycerate kinase (PGK)
Förster resonance energy transfer (FRET) constructs localized in the nucleus or in the …
Förster resonance energy transfer (FRET) constructs localized in the nucleus or in the …
Spectroscopic studies on binding of ibuprofen and drotaverine with bovine serum albumin
The interaction between ibuprofen (analgesic) and drotaverine (smooth muscle relaxant)
drugs with bovine serum albumin (BSA) was investigated using both experimental (UV–Vis …
drugs with bovine serum albumin (BSA) was investigated using both experimental (UV–Vis …
Cell-free protein expression under macromolecular crowding conditions
Background Cell-free protein expression (CFPE) comprised of in vitro transcription and
translation is currently manipulated in relatively dilute solutions, in which the …
translation is currently manipulated in relatively dilute solutions, in which the …