The structure and function of the eukaryotic ribosome
Structures of the bacterial ribosome have provided a framework for understanding universal
mechanisms of protein synthesis. However, the eukaryotic ribosome is much larger than it is …
mechanisms of protein synthesis. However, the eukaryotic ribosome is much larger than it is …
Arrest Peptides: Cis-Acting Modulators of Translation
K Ito, S Chiba - Annual review of biochemistry, 2013 - annualreviews.org
Each peptide bond of a protein is generated at the peptidyl transferase center (PTC) of the
ribosome and then moves through the exit tunnel, which accommodates ever-changing …
ribosome and then moves through the exit tunnel, which accommodates ever-changing …
Translation rate is controlled by coupled trade-offs between site accessibility, selective RNA unfolding and sliding at upstream standby sites
A Espah Borujeni, AS Channarasappa… - Nucleic acids …, 2014 - academic.oup.com
The ribosome's interactions with mRNA govern its translation rate and the effects of post-
transcriptional regulation. Long, structured 5′ untranslated regions (5′ UTRs) are …
transcriptional regulation. Long, structured 5′ untranslated regions (5′ UTRs) are …
Measurements of translation initiation from all 64 codons in E. coli
Our understanding of translation underpins our capacity to engineer living systems. The
canonical start codon (AUG) and a few near-cognates (GUG, UUG) are considered as the …
canonical start codon (AUG) and a few near-cognates (GUG, UUG) are considered as the …
Bacterial protein synthesis as a target for antibiotic inhibition
Protein synthesis occurs on macromolecular machines, called ribosomes. Bacterial
ribosomes and the translational machinery represent one of the major targets for antibiotics …
ribosomes and the translational machinery represent one of the major targets for antibiotics …
Precise quantification of translation inhibition by mRNA structures that overlap with the ribosomal footprint in N-terminal coding sequences
A Espah Borujeni, D Cetnar, I Farasat… - Nucleic acids …, 2017 - academic.oup.com
A mRNA's translation rate is controlled by several sequence determinants, including the
presence of RNA structures within the N-terminal regions of its coding sequences. However …
presence of RNA structures within the N-terminal regions of its coding sequences. However …
Superfolder GFP reporters validate diverse new mRNA targets of the classic porin regulator, MicF RNA
CP Corcoran, D Podkaminski, K Papenfort… - Molecular …, 2012 - Wiley Online Library
MicF is a textbook example of a small regulatory RNA (sRNA) that acts on a trans‐encoded
target mRNA through imperfect base pairing. Discovery of MicF as a post‐transcriptional …
target mRNA through imperfect base pairing. Discovery of MicF as a post‐transcriptional …
Translation initiation is controlled by RNA folding kinetics via a ribosome drafting mechanism
A Espah Borujeni, HM Salis - Journal of the American Chemical …, 2016 - ACS Publications
RNA folding plays an important role in controlling protein synthesis as well as other cellular
processes. Existing models have focused on how RNA folding energetics control translation …
processes. Existing models have focused on how RNA folding energetics control translation …
The universally conserved prokaryotic GTPases
Members of the large superclass of P-loop GTPases share a core domain with a conserved
three-dimensional structure. In eukaryotes, these proteins are implicated in various crucial …
three-dimensional structure. In eukaryotes, these proteins are implicated in various crucial …
Escherichia coli Ribosomal Protein S1 Unfolds Structured mRNAs Onto the Ribosome for Active Translation Initiation
M Duval, A Korepanov, O Fuchsbauer, P Fechter… - PLoS …, 2013 - journals.plos.org
Regulation of translation initiation is well appropriate to adapt cell growth in response to
stress and environmental changes. Many bacterial mRNAs adopt structures in their 5 …
stress and environmental changes. Many bacterial mRNAs adopt structures in their 5 …