Single-nucleus cross-tissue molecular reference maps toward understanding disease gene function

G Eraslan, E Drokhlyansky, S Anand, E Fiskin… - Science, 2022 - science.org
Understanding gene function and regulation in homeostasis and disease requires
knowledge of the cellular and tissue contexts in which genes are expressed. Here, we …

[HTML][HTML] Microneedle-based interstitial fluid extraction for drug analysis: Advances, challenges, and prospects

S Ma, J Li, L Pei, N Feng, Y Zhang - Journal of Pharmaceutical Analysis, 2023 - Elsevier
Similar to blood, interstitial fluid (ISF) contains exogenous drugs and biomarkers and may
therefore substitute blood in drug analysis. However, current ISF extraction techniques …

Developmental cell programs are co-opted in inflammatory skin disease

G Reynolds, P Vegh, J Fletcher, EFM Poyner… - Science, 2021 - science.org
INTRODUCTION Human skin provides vital protection from water loss and from external
insult through structural adaptations and interplay with the innate and adaptive immune …

Dysregulation of the epithelial barrier by environmental and other exogenous factors

Y Mitamura, I Ogulur, Y Pat, AO Rinaldi… - Contact …, 2021 - Wiley Online Library
The “epithelial barrier hypothesis” proposes that the exposure to various epithelial barrier–
damaging agents linked to industrialization and urbanization underlies the increase in …

Classification of human chronic inflammatory skin disease based on single-cell immune profiling

Y Liu, H Wang, M Taylor, C Cook… - Science …, 2022 - science.org
Inflammatory conditions represent the largest class of chronic skin disease, but the
molecular dysregulation underlying many individual cases remains unclear. Single-cell RNA …

TREM2 macrophages induced by human lipids drive inflammation in acne lesions

TH Do, F Ma, PR Andrade, R Teles… - Science …, 2022 - science.org
Acne affects 1 in 10 people globally, often resulting in disfigurement. The disease involves
excess production of lipids, particularly squalene, increased growth of Cutibacterium acnes …

Proximity extension assay in combination with next-generation sequencing for high-throughput proteome-wide analysis

L Wik, N Nordberg, J Broberg, J Björkesten… - Molecular & Cellular …, 2021 - ASBMB
Understanding the dynamics of the human proteome is crucial for develo** biomarkers to
be used as measurable indicators for disease severity and progression, patient stratification …

Homeostatic IL-13 in healthy skin directs dendritic cell differentiation to promote TH2 and inhibit TH17 cell polarization

JU Mayer, KL Hilligan, JS Chandler, DA Eccles… - Nature …, 2021 - nature.com
The signals driving the adaptation of type 2 dendritic cells (DC2s) to diverse peripheral
environments remain mostly undefined. We show that differentiation of CD11blo migratory …

Persistence of mature dendritic cells, TH2A, and Tc2 cells characterize clinically resolved atopic dermatitis under IL-4Rα blockade

C Bangert, K Rindler, T Krausgruber, N Alkon… - Science …, 2021 - science.org
Therapeutic options for autoimmune diseases typically consist of broad and targeted
immunosuppressive agents. However, sustained clinical benefit is rarely achieved, as the …

scRNA-seq of human vitiligo reveals complex networks of subclinical immune activation and a role for CCR5 in Treg function

KJ Gellatly, JP Strassner, K Essien, MA Refat… - Science translational …, 2021 - science.org
Vitiligo is an autoimmune skin disease characterized by the targeted destruction of
melanocytes by T cells. Cytokine signaling between keratinocytes and T cells results in …