Rapid and accurate molecular diagnosis is a prerequisite for precision medicine, food
safety, and environmental monitoring. The clustered regularly interspaced short palindromic …

Nucleic acid cleavage processes contribute to target and signal amplification, which is
critical for the development of highly sensitive assays. 1, 2 Exonucleases or endonucleases …

Nucleic acid molecules possess many superior properties, including high designability and
structural predictability, making them attractive biosensing and bioimaging tools. The …

The trans-cleavage activity of Cas12a has been widely used with various applications. Here,
we report that the trans-cleavage activity of Cas12a can be significantly affected by the …

Control of CRISPR/Cas12a trans-cleavage is crucial for biosensor development. Here, we
show that small circular DNA nanostructures which partially match guide RNA sequences …

The trans‐cleavage property of CRISPR‐Cas12a system makes it an excellent tool for
disease diagnosis. Nevertheless, most methods based on CRISPR‐Cas system still require …

CRISPR/Cas12a has been believed to be powerful in molecular detection and diagnostics
due to its amplified trans-cleavage feature. However, the activating specificity and multiple …

Background Early detection of pathogen-associated diseases are critical for effective
treatment. Rapid, specific, sensitive, and cost-effective diagnostic technologies continue to …

Biosensing based on CRISPR-Cas systems is a young but rapidly evolving technology. The
unprecedented properties of the CRISPR-Cas system provide an innovative tool for …

Sepsis is an extremely dangerous medical condition that emanates from the body's
response to a pre-existing infection. Early detection of sepsis-inducing bacterial infections …