The emergence of CRISPR-Cas systems has accelerated the development of gene editing
technologies, which are widely used in the life sciences. To improve the performance of …

The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …

RNA-guided programmable nucleases from CRISPR systems generate precise breaks in
DNA or RNA at specified positions. In cells, this activity can lead to changes in DNA …

Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are
expressed at once, have facilitated powerful biological engineering applications, vastly …

DNA is naturally well suited to serve as a digital medium for in vivo molecular recording.
However, contemporary DNA-based memory devices are constrained in terms of the …

Genetically modified microorganisms (GMMs) can enable a wide range of important
applications including environmental sensing and responsive engineered living materials …

Rapid advances in synthetic biology have fueled interest in engineered microorganisms that
can diagnose and treat disease. However, designing bacteria that detect dynamic disease …

Genome editing with CRISPR/Cas has rapidly gained popularity. Base editing, a new
CRISPR/Cas-based approach, can precisely convert one nucleotide to another in DNA or …

Genome editing has transformed the life sciences and has exciting prospects for use in
treating genetic diseases. Our laboratory developed base editing to enable precise and …

Measurements of gene expression or signal transduction activity are conventionally
performed using methods that require either the destruction or live imaging of a biological …