Agrobacterium-mediated plant transformation: biology and applications

HH Hwang, M Yu, EM Lai - The arabidopsis book, 2017 - pmc.ncbi.nlm.nih.gov
Plant genetic transformation heavily relies on the bacterial pathogen Agrobacterium
tumefaciens as a powerful tool to deliver genes of interest into a host plant. Inside the plant …

A comprehensive review of in planta stable transformation strategies

JG Bélanger, TR Copley, V Hoyos-Villegas, JB Charron… - Plant Methods, 2024 - Springer
Plant transformation remains a major bottleneck to the improvement of plant science, both
on fundamental and practical levels. The recalcitrant nature of most commercial and minor …

Agrobacterium rhizogenes-Transformed Roots of Medicago truncatula for the Study of Nitrogen-Fixing and Endomycorrhizal Symbiotic Associations

A Boisson-Dernier, M Chabaud… - Molecular Plant …, 2001 - Am Phytopath Society
Medicago truncatula, a diploid autogamous legume, is currently being developed as a
model plant for the study of root endosymbiotic associations, including nodulation and …

Optimization of Agrobacterium‐mediated transient assays of gene expression in lettuce, tomato and Arabidopsis

T Wroblewski, A Tomczak… - Plant biotechnology …, 2005 - Wiley Online Library
Agrobacterium‐mediated transient assays for gene function are increasingly being used as
alternatives to genetic complementation and stable transformation. However, such assays …

Achievements and challenges in legume breeding for pest and disease resistance

D Rubiales, S Fondevilla, W Chen… - Critical Reviews in …, 2015 - Taylor & Francis
Yield stability of legume crops is constrained by a number of pest and diseases. Major
diseases are rusts, powdery and downy mildews, ascochyta blights, botrytis gray molds …

A chloroplast phosphate transporter, PHT2; 1, influences allocation of phosphate within the plant and phosphate-starvation responses

WK Versaw, MJ Harrison - The Plant Cell, 2002 - academic.oup.com
The uptake and distribution of Pi in plants requires multiple Pi transport systems that must
function in concert to maintain homeostasis throughout growth and development. The Pi …

An in planta biolistic method for stable wheat transformation

H Hamada, Q Linghu, Y Nagira, R Miki, N Taoka… - Scientific reports, 2017 - nature.com
The currently favoured method for wheat (Triticum aestivum L.) transformation is
inapplicable to many elite cultivars because it requires callus culture and regeneration …

Recent advances in legume transformation

DA Somers, DA Samac, PM Olhoft - Plant physiology, 2003 - academic.oup.com
Legumes are a large, diverse family ranging from herbaceous annuals to woody perennials
that, because of their capacity to fix nitrogen, are essential components in natural and …

A Phosphate Transporter Gene from the Extra-Radical Mycelium of an Arbuscular Mycorrhizal Fungus Glomus intraradices Is Regulated in Response to Phosphate in …

IE Maldonado-Mendoza, GR Dewbre… - Molecular Plant …, 2001 - Am Phytopath Society
The majority of vascular flowering plants are able to form symbiotic associations with
arbuscular mycorrhizal fungi. These symbioses, termed arbuscular mycorrhizas, are …

Arabidopsis thaliana Floral Dip Transformation Method

A Bent - Agrobacterium protocols, 2006 - Springer
Arabidopsis floral dip transformation is notable for a number of reasons. First, it is strikingly
simple to perform. Agrobacterium is applied to flowering Arabidopsis plants that …