Towards real-time image deconvolution: application to confocal and STED microscopy

R Zanella, G Zanghirati, R Cavicchioli, L Zanni… - Scientific reports, 2013 - nature.com
Although deconvolution can improve the quality of any type of microscope, the high
computational time required has so far limited its massive spreading. Here we demonstrate …

Spatial filter based Bessel-like beam for improved penetration depth imaging in fluorescence microscopy

SB Purnapatra, S Bera, PP Mondal - Scientific reports, 2012 - nature.com
Monitoring and visualizing specimens at a large penetration depth is a challenge. At depths
of hundreds of microns, several physical effects (such as, scattering, PSF distortion and …

[HTML][HTML] Multi-images deconvolution improves signal-to-noise ratio on gated stimulated emission depletion microscopy

M Castello, A Diaspro, G Vicidomini - Applied Physics Letters, 2014 - pubs.aip.org
Time-gated detection, namely, only collecting the fluorescence photons after a time-delay
from the excitation events, reduces complexity, cost, and illumination intensity of a stimulated …

Fluorescence-based multifunctional light sheet imaging flow cytometry for high-throughput optical interrogation of live cells

P Joshi, P Kumar, JM Varghese, PP Mondal - Communications Physics, 2024 - nature.com
Multifunctional light sheet imaging flow cytometry of a large population of live cells at high
throughput is challenging and requires new technological advancement. Existing cytometry …

Integrated light-sheet imaging and flow-based enquiry (iLIFE) system for 3D in-vivo imaging of multicellular organism

CK Rasmi, S Padmanabhan, K Shirlekar… - Applied Physics …, 2017 - pubs.aip.org
We propose and demonstrate a light-sheet-based 3D interrogation system on a microfluidic
platform for screening biological specimens during flow. To achieve this, a diffraction-limited …

Single-molecule clustering for super-resolution optical fluorescence microscopy

P Joshi, PP Mondal - Photonics, 2021 - mdpi.com
Molecular assembly in a complex cellular environment is vital for understanding underlying
biological mechanisms. Biophysical parameters (such as single-molecule cluster density …

Application of the split‐gradient method to 3D image deconvolution in fluorescence microscopy

G Vicidomini, P Boccacci, A Diaspro… - Journal of …, 2009 - Wiley Online Library
The methods of image deconvolution are important for improving the quality of the detected
images in the different modalities of fluorescence microscopy such as wide‐field, confocal …

Image reconstruction for multiphoton fluorescence microscopy

PP Mondal, G Vicidomini, A Diaspro - Applied Physics Letters, 2008 - pubs.aip.org
We present a general image reconstruction methodology for multiphoton fluorescence
microscopy. The method is primarily based on Markov random field modeling of the detected …

A holistic approach for role inference and action anticipation in human teams

J Dong, Q Huo, S Ferrari - ACM Transactions on Intelligent Systems and …, 2022 - dl.acm.org
The ability to anticipate human actions is critical to many cyber-physical systems, such as
robots and autonomous vehicles. Computer vision and sensing algorithms to date have …

Application of the maximum flow–minimum cut algorithm to segmentation and clustering of materials datasets

AF Brust, EJ Payton, TJ Hobbs… - Microscopy and …, 2019 - cambridge.org
Problems involving image segmentation, atomic cluster identification, segmentation of
microstructure constituents in images and austenite reconstruction have seen various …