Many diseases are related to abnormal metabolites in cells. It is of great significance to
analyze the metabolites for cellular physiological, pathological, and pharmacological …

MS imaging (MSI) is a powerful tool in drug discovery because of its ability to interrogate a
wide range of endogenous and exogenous molecules in a broad variety of samples. The …

Accurately monitoring H 2 O 2 concentrations in 3D cell clusters is challenging due to limited
diffusion and rapid degradation of H 2 O 2 in the culture medium. Despite the incorporation …

Cellular heterogeneity is generally overlooked in infectious diseases. In this study, we
investigated host cell heterogeneity during infection with Trypanosoma cruzi (T. cruzi) …

The exploration of single cells reveals cell heterogeneity and biological principle of cellular
metabolism. Although a number of mass spectrometry (MS) based single cell MS (SCMS) …

Cancer stem cells (CSCs) are rare types of cells responsible for tumor development,
relapse, and metastasis. However, current research in CSC biology is largely limited by the …

The application of mass spectrometry imaging (MSI) to explore the responses of cancer cell
spheroids (CCS) after treatment of exogenous molecules has attracted growing attention …

Spatially resolved metabolomics offers unprecedented opportunities for elucidating
metabolic mechanisms during cancer progression. It facilitated the discovery of aberrant …

Abstract Three-dimensional (3D) multicellular tumor spheroids (MCTS) that mimic the
complex tumor microenvironment provide a good platform for in vitro study of drug and …

Altered lipid metabolism is one of the hallmarks of cancer. Cellular proliferation and de novo
synthesis of lipids are related to cancer progression. In this study, we evaluated the lipidomic …