Recent advances in microbial ecology and synthetic biology have the potential to mitigate
damage caused by anthropogenic activities that are deleteriously impacting Earth's soil …

Climate crisis and rapid population growth are posing some of the most urgent challenges to
mankind and have intensified the need for the deployment of carbon recycling and carbon …

Understanding microbial gene functions relies on the application of experimental genetics in
cultured microorganisms. However, the vast majority of bacteria and archaea remain …

While there are several genome editing techniques available, few are suitable for dynamic
and simultaneous mutagenesis of arbitrary targeted sequences in prokaryotes. Here, to …

The molecular and ecological factors sha** horizontal gene transfer (HGT) via natural
transformation in microbial communities are largely unknown, which is critical for …

Traditional microbial biotechnology is in the midst of a profound transformation brought
about not only by many conceptual and technical breakthroughs (eg systems and synthetic …

CRISPR–Cas systems have revolutionized genome editing across a broad range of
biotechnological endeavors. Many CRISPR-Cas nucleases have been identified and …

The advances in synthetic biology bring exciting new opportunities to reprogram
microorganisms with novel functionalities for environmental applications. For real‐world …

Chromosomal recombinant gene expression offers a number of advantages over plasmid-
based synthetic biology. However, the methods applied for bacterial genome engineering …

Development of profiling strategies to provide high resolution understanding of enzymes
involved in bacterial infections remains an important need. These strategies help resolve …