Gene regulatory network inference in the era of single-cell multi-omics

P Badia-i-Mompel, L Wessels, S Müller-Dott… - Nature Reviews …, 2023 - nature.com
The interplay between chromatin, transcription factors and genes generates complex
regulatory circuits that can be represented as gene regulatory networks (GRNs). The study …

3D enhancer-promoter interactions and multi-connected hubs: Organizational principles and functional roles

CM Uyehara, E Apostolou - Cell reports, 2023 - cell.com
The spatiotemporal control of gene expression is dependent on the activity of cis-acting
regulatory sequences, called enhancers, which regulate target genes over variable genomic …

Super-resolution chromatin tracing reveals domains and cooperative interactions in single cells

B Bintu, LJ Mateo, JH Su, NA Sinnott-Armstrong… - Science, 2018 - science.org
INTRODUCTION Chromatin adopts an intricate three-dimensional (3D) organization in the
nucleus that is critical for many genome processes, from gene regulation to genome …

Promoter-proximal CTCF binding promotes distal enhancer-dependent gene activation

N Kubo, H Ishii, X **ong, S Bianco… - Nature structural & …, 2021 - nature.com
The CCCTC-binding factor (CTCF) works together with the cohesin complex to drive the
formation of chromatin loops and topologically associating domains, but its role in gene …

Joint profiling of histone modifications and transcriptome in single cells from mouse brain

C Zhu, Y Zhang, YE Li, J Lucero, MM Behrens, B Ren - Nature methods, 2021 - nature.com
Genome-wide profiling of histone modifications can reveal not only the location and activity
state of regulatory elements, but also the regulatory mechanisms involved in cell-type …

Mitochondrial base editor induces substantial nuclear off-target mutations

Z Lei, H Meng, L Liu, H Zhao, X Rao, Y Yan, H Wu… - Nature, 2022 - nature.com
DddA-derived cytosine base editors (DdCBEs)—which are fusions of split DddA halves and
transcription activator-like effector (TALE) array proteins from bacteria—enable targeted C• …

A compendium of promoter-centered long-range chromatin interactions in the human genome

I Jung, A Schmitt, Y Diao, AJ Lee, T Liu, D Yang… - Nature …, 2019 - nature.com
A large number of putative cis-regulatory sequences have been annotated in the human
genome, but the genes they control remain poorly defined. To bridge this gap, we generate …

An ultra high-throughput method for single-cell joint analysis of open chromatin and transcriptome

C Zhu, M Yu, H Huang, I Juric, A Abnousi… - Nature structural & …, 2019 - nature.com
Simultaneous profiling of transcriptome and chromatin accessibility within single cells is a
powerful approach to dissect gene regulatory programs in complex tissues. However …

Transcriptionally active HERV-H retrotransposons demarcate topologically associating domains in human pluripotent stem cells

Y Zhang, T Li, S Preissl, ML Amaral, JD Grinstein… - Nature …, 2019 - nature.com
Chromatin architecture has been implicated in cell type-specific gene regulatory programs,
yet how chromatin remodels during development remains to be fully elucidated. Here, by …

Structural variants drive context-dependent oncogene activation in cancer

Z Xu, DS Lee, S Chandran, VT Le, R Bump, J Yasis… - Nature, 2022 - nature.com
Higher-order chromatin structure is important for the regulation of genes by distal regulatory
sequences,. Structural variants (SVs) that alter three-dimensional (3D) genome organization …