Ras conformational ensembles, allostery, and signaling
Ras proteins are classical members of small GTPases that function as molecular switches by
alternating between inactive GDP-bound and active GTP-bound states. Ras activation is …
alternating between inactive GDP-bound and active GTP-bound states. Ras activation is …
The K-Ras, N-Ras, and H-Ras isoforms: unique conformational preferences and implications for targeting oncogenic mutants
Ras controls a multitude of cellular signaling processes, including cell proliferation,
differentiation, and apoptosis. Deregulation of Ras cycling often promotes tumorigenesis …
differentiation, and apoptosis. Deregulation of Ras cycling often promotes tumorigenesis …
Motorless transport of microtubules along tubulin, RanGTP, and salt gradients
Microtubules are dynamic filaments that assemble spindles for eukaryotic cell division. As
the concentration profiles of soluble tubulin and regulatory proteins are non-uniform during …
the concentration profiles of soluble tubulin and regulatory proteins are non-uniform during …
Structures of N-terminally processed KRAS provide insight into the role of N-acetylation
Although post-translational modification of the C-terminus of RAS has been studied
extensively, little is known about N-terminal processing. Mass spectrometric characterization …
extensively, little is known about N-terminal processing. Mass spectrometric characterization …
K-Ras populates conformational states differently from its isoform H-Ras and oncogenic mutant K-RasG12D
Structures of wild-type K-Ras from crystals obtained in the presence of guanosine
triphosphate (GTP) or its analogs have remained elusive. Of the K-Ras mutants, only K …
triphosphate (GTP) or its analogs have remained elusive. Of the K-Ras mutants, only K …
Isothermal chemical denaturation assay for monitoring protein stability and inhibitor interactions
R Mahran, N Vello, A Komulainen, M Malakoutikhah… - Scientific Reports, 2023 - nature.com
Thermal shift assay (TSA) with altered temperature has been the most widely used method
for monitoring protein stability for drug research. However, there is a pressing need for …
for monitoring protein stability for drug research. However, there is a pressing need for …
GTP hydrolysis without an active site base: a unifying mechanism for Ras and related GTPases
GTP hydrolysis is a biologically crucial reaction, being involved in regulating almost all
cellular processes. As a result, the enzymes that catalyze this reaction are among the most …
cellular processes. As a result, the enzymes that catalyze this reaction are among the most …
Ras and GTPase-activating protein (GAP) drive GTP into a precatalytic state as revealed by combining FTIR and biomolecular simulations
Members of the Ras superfamily regulate many cellular processes. They are down-
regulated by a GTPase reaction in which GTP is cleaved into GDP and P i by nucleophilic …
regulated by a GTPase reaction in which GTP is cleaved into GDP and P i by nucleophilic …
Hydrolysis of guanosine triphosphate (GTP) by the Ras· GAP protein complex: reaction mechanism and kinetic scheme
MG Khrenova, BL Grigorenko… - The Journal of …, 2015 - ACS Publications
Molecular mechanisms of the hydrolysis of guanosine triphosphate (GTP) to guanosine
diphosphate (GDP) and inorganic phosphate (Pi) by the Ras· GAP protein complex are fully …
diphosphate (GDP) and inorganic phosphate (Pi) by the Ras· GAP protein complex are fully …
Identification of functional substates of KRas during GTP hydrolysis with enhanced sampling simulations
As the hub of major signaling pathways, Ras proteins are implicated in 19% of tumor-caused
cancers due to perturbations in their conformational and/or catalytic properties. Despite …
cancers due to perturbations in their conformational and/or catalytic properties. Despite …