Advances and challenges in biotechnological production of chondroitin sulfate and its oligosaccharides

W Zhang, R Xu, J Chen, H **ong, Y Wang… - International journal of …, 2023‏ - Elsevier
Chondroitin sulfate (CS) is a member of glycosaminoglycans (GAGs) and has critical
physiological functions. CS is widely applied in medical and clinical fields. Currently, the …

Biotechnological advances in the synthesis of modified chondroitin towards novel biomedical applications

D Cimini, E Bedini, C Schiraldi - Biotechnology Advances, 2023‏ - Elsevier
Chondroitin sulfate (CS) is a well-known glycosaminoglycan present in a large variety of
animal tissues, with an outstanding structural heterogeneity mainly related to molecular …

Imaging of Escherichia coli K5 and glycosaminoglycan precursors via targeted metabolic labeling of capsular polysaccharides in bacteria

YJ Wang, L Li, J Yu, HY Hu, ZX Liu, WJ Jiang, W Xu… - Science …, 2023‏ - science.org
The introduction of unnatural chemical moieties into glycosaminoglycans (GAGs) has
enormous potential to facilitate studies of the mechanism and application of these critical …

[HTML][HTML] Heterologous production of chondroitin

MR Couto, JL Rodrigues, LR Rodrigues - Biotechnology Reports, 2022‏ - Elsevier
Chondroitin sulfate (CS) is a glycosaminoglycan with a broad range of applications being a
popular dietary supplement for osteoarthritis. Usually, CS is extracted from animal sources …

Enhancing the expression of chondroitin 4-O-sulfotransferase for one-pot enzymatic synthesis of chondroitin sulfate A

W Zhang, P Zhang, H Wang, R Xu, Z **e, Y Wang… - Carbohydrate …, 2024‏ - Elsevier
Chondroitin sulfate (CS) stands as a pivotal compound in dietary supplements for
osteoarthritis treatment, propelling significant interest in the biotechnological pursuit of …

[HTML][HTML] Characterization of emergent Avibacterium paragallinarum strains and the protection conferred by infectious coryza vaccines against them in China

Y Xu, J Cheng, X Huang, M Xu, J Feng, C Liu, G Zhang - Poultry Science, 2019‏ - Elsevier
Infectious coryza (IC), an acute respiratory disease of chickens, is caused by Avibacterium
paragallinarum. Here, the current epidemiological status of IC was investigated in China …

Cell-based assay approaches for glycosaminoglycan synthase high-throughput screening: development and applications

HY Hu, JQ Deng, YJ Wang, Y Li, XL Meng… - ACS Chemical …, 2023‏ - ACS Publications
Glycosaminoglycan synthases have immense potential in applications involving synthesis of
oligosaccharides, using enzymatic approaches and construction of cell factories that …

Microbial production and metabolic engineering of chondroitin and chondroitin sulfate

D Cimini, OF Restaino, C Schiraldi - Emerging Topics in Life …, 2018‏ - portlandpress.com
Several commercial uses and potential novel applications have recently been described for
chondroitin sulfate (CS). However, the currently applied animal extractive procedure has a …

Novel β1,4 N-acetylglucosaminyltransferase in de novo enzymatic synthesis of hyaluronic acid oligosaccharides

JY Sun, JQ Deng, RR Du, SY **n, YL Cao, Z Lu… - Applied Microbiology …, 2023‏ - Springer
The efficiency of de novo synthesis of hyaluronic acid (HA) using Pasteurella multocida
hyaluronate synthase (PmHAS) is limited by its low catalytic activity during the initial reaction …

Identification of a novel glucuronyltransferase from Streptomyces chromofuscus ATCC 49982 for natural product glucuronidation

J Ren, CD Barton, KE Sorenson, J Zhan - Applied Microbiology and …, 2022‏ - Springer
Glycosylation is an effective way to increase the polarity of natural products. UDP-
glucuronyltransferases (UGTs) are commonly observed and extensively studied in phase II …