Single-molecule localization microscopy (SMLM) describes a family of powerful imaging
techniques that dramatically improve spatial resolution over standard, diffraction-limited …

Super-resolution microscopy techniques, and specifically single-molecule localization
microscopy (SMLM), are approaching nanometer resolution inside cells and thus have great …

Although StayGold is a bright and highly photostable fluorescent protein, its propensity for
obligate dimer formation may hinder applications in molecular fusion and membrane …

Fluorescence microscopy has become a ubiquitous method to observe the location of
specific molecular components within cells. However, the resolution of light microscopy is …

Fluorescence nanoscopy, or super-resolution microscopy, has become an important tool in
cell biological research. However, because of its usually inferior resolution in the depth …

The spatial resolution of fluorescence microscopy has recently been greatly enhanced.
However, improvements in temporal resolution have been limited, despite their importance …

Imaging cellular structures and organelles in living cells by long time-lapse super-resolution
microscopy is challenging, as it requires dense labeling, bright and highly photostable dyes …

High laser powers are common practice in single-molecule localization microscopy to speed
up data acquisition. Here we systematically quantified how excitation intensity influences …

Super-resolution optical imaging based on the switching and localization of individual
fluorescent molecules [photoactivated localization microscopy (PALM), stochastic optical …

Using our newly developed ultrafast camera described in the companion paper, we reduced
the data acquisition periods required for photoactivation/photoconversion localization …